摘要
采用沉淀转化法制备出粒径约为10 nm的氧化铜纳米颗粒(CuO NPs),通过盐酸多巴胺(DA HCl)在其表面进行聚合,并通过叶酸(FA)修饰形成CuO@PDA-FA NPs。采用透射电子显微镜、红外光谱仪、纳米粒径电势分析仪、X射线光电子能谱对纳米颗粒的形貌和性质进行表征。结果表明,制备的CuO@PDA-FA NPs粒径约为190 nm。材料降解亚甲基蓝试验和氧化葡萄糖试验证实CuO@PDA-FA NPs具备类过氧化物酶(POD)活性和类葡萄糖氧化酶(GOD)活性。近红外光热转换试验证实CuO@PDA-FA NPs具有良好的光热效应和光热稳定性。体外细胞毒性试验、细胞摄取试验和活性氧生成试验结果表明,CuO@PDA-FA NPs能主动靶向肿瘤细胞,并通过光热治疗(PTT)/化学动力学治疗(CDT)/饥饿治疗(ST)协同效果杀伤肿瘤细胞。
Copper oxide nanoparticles(CuO NPs)with the size of about 10 nm were prepared by precipitation transformation method,and CuO@PDA-FA NPs were synthesized by polymerization of dopamine hydrochloride(DA HCl)and modification of folic acid(FA)on its surface.The morphology and properties of the nanoparticles were characterized by transmission electron microscope,infrared spectrometer,nano-particle size potential analyzer and X-ray photoelectron spectroscopy.The results show that CuO@PDA-FA NPs with a particle size of about 190 nm are prepared.The degradation of methylene blue and glucose oxide confirmed that the material has peroxidase(POD)activity and glucose oxidase(GOD)activity.Near-infrared photothermal conversion experiments show that it has good photothermal effect and photothermal stability.The results of in vitro cytotoxicity test,cell uptake test and reactive oxygen generation test show that CuO@PDA-FA NPs can actively target tumor cells and kill tumor cells through the synergistic effect of photothermal therapy(PTT),chemical dynamic therapy(CDT)and starvation therapy(ST).
作者
邓凯夫
郑轶璐
王通
郑之裕
修梦婷
任蓉
VAN CUONG Nguyen
朱利民
DENG Kaifu;ZHENG Yilu;WANG Tong;ZHENG Zhiyu;XIU Mengting;REN Rong;VAN CUONG Nguyen;ZHU Limin(College of Biological Science and Medical Engineering,Donghua University,Shanghai,China;Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,Donghua University,Shanghai,China;Faculty of Chemical Engineering,Industrial University of Ho Chi Minh City,Ho Chi Minh,Vietnam)
出处
《东华大学学报（自然科学版）》
CAS
北大核心
2024年第2期59-69,共11页
Journal of Donghua University(Natural Science)
基金
上海市科学技术委员会资助项目(22520710400,21WZ2501300,20DZ2254900)
中国教育部生物医用纺织材料“111工程”(B07024)。
关键词
氧化铜
纳米颗粒
叶酸
聚多巴胺
化学动力学治疗
光热治疗
模拟酶
copper oxide
nanoparticle
folic acid
polydopamine
chemodynamic therapy
photothermal therapy
mimic enzyme
