摘要
建立一种QuEChERS-超高效液相色谱-串联质谱法同时测定茶叶中47种农药残留的分析方法。样品经1%乙酸乙腈提取,QuEChERS方法净化,配有电喷雾离子源(ESI±)的超高效液相色谱-串联质谱(UPLC-MS/MS),在正负离子多反应监测(MRM)模式下同时测定,基质匹配外标法定量。结果表明:47种目标化合物在一定范围内线性关系良好,相关系数(r)为0.9971~0.9996,检出限为0.001~0.006 mg/kg,定量限为0.002~0.020 mg/kg,平均回收率为73.4%~114.3%,相对标准偏差(n=6)为0.3%~19.9%。该方法灵敏、准确、稳定,可满足茶叶中47种农药残留的检测要求。
An analysis method for simultaneous determination of 47 kinds of pesticide residues in tea was established by QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry.The samples were extracted by acetonitrile containing 1%acetic acid,and purified by QuEChERS method.The 47 pesticides were determined by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)with an electrospray ion source(ESI±)in positive and negative ion switching multiple reaction monitoring(MRM),and quantified by matrix-matched external standard method.Method validation revealed satisfactory linearities with correlation coefficients(r)between 0.9971 and 0.9996,and limits of detection were 0.001~0.006 mg/kg while the limits of quantitation were 0.002~0.020 mg/kg,the average recoveries at three spiked levels were in the range of 73.4%~114.3%,with relative standard deviations(n=6)of 0.3%~19.9%.The method is sensitive,accurate,stable,and can meet the requirements for determination of 47 pesticide residues in tea.
作者
刘华文
邓美晴
陈北燕
LIU Huawen;DENG Meiqing;CHEN Beiyan(Guangxi-Asean Food Inspection Center,Nanning 530029,China;School of Public Health,Guangxi Medical University,Nanning 530022,China;Nanning Health School,Nanning 530409,China)
出处
《食品工业科技》
CAS
北大核心
2024年第9期255-264,共10页
Science and Technology of Food Industry
基金
植物源性食品中植物生长调节剂风险筛查研究(GXSJKJ2023-18)。
