摘要
【目的】核糖体蛋白(ribosomal protein,RP)是参与蛋白质合成及基因表达调控的一种重要因子,在植物生长发育和胁迫响应过程中具有重要的作用。研究在水稻中克隆了1个核糖体蛋白家族基因OsRPL36A,并对其生物学功能进行初步分析,为后续OsRPL36A基因功能研究提供理论依据。【方法】用生物信息学技术分析OsRPL36A基因结构、顺式作用元件和演化过程;用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)技术分析OsRPL36A的组织表达特异性、节律表达模式、及其对不同激素和非生物胁迫的响应情况。【结果】(1)OsRPL36A的编码区全长为297 bp,共编码98个氨基酸,属于核糖体蛋白L36超基因家族。(2)OsRPL36A的启动子区包含3个节律表达相关元件、10个光响应元件、14个激素响应元件和27个环境胁迫响应元件。(3)OsRPL36A在叶片中的表达量相对高于其他组织;具有典型的节律表达模式;且受IAA、高温、低温和渗透胁迫等诱导表达。【结论】OsRPL36A在叶中高表达,具有典型节律表达模式,对IAA显著响应,可能参与热激、低温、盐害和渗透胁迫响应。
[Objective]As an important regulator involved in protein synthesis and gene expression,ribosomal proteins are crucial for plant growth and development and stress response.In this study,a ribosomal protein family gene OsRPL36A was cloned from rice,and a preliminary study was performed for its biological function,providing a basis for the study of OsRPL36A function.[Methods]Bioinformatics techniques were used to analysis gene structure,cis-regulatory elements,and evolution process of OsRPL36A.Meanwhile,the expression of OsRPL36A in different tissues and under diurnal rhythm,different hormones,and abiotic stresses were analyzed by quantitative real-time PCR(qRT-PCR).[Results](1)OsRPL36A encoded 98 amino acids with a 297 bp coding region,which belonged to the ribosomal protein L36 supergene family,and was named as OsRPL36A.(2)The promoter region of OsRPL36A contained 3 rhythmic expression-related elements,10 light-responsive elements,14 hormone-responsive elements,and 27 environmental stress response elements.(3)The expression of OsRPL36A in leaves was relatively higher than that in other tissues and presented a typical rhythmic expression pattern.The expression of OsRPL36A was induced by IAA,high temperature,low temperature,osmotic stress,and so on.[Conclusion]OsRPL36A was highly expressed in leaves and presented a typical rhythmic expression pattern.OsRPL36A responded to IAA and might be involved in response to heat shock,low temperature,salt,and osmotic stresses.
作者
崔桌铖
李乐晨
范金岚
曾媛欢
李克克
唐辉武
CUI Zhuocheng;LI Yuechen;FAN Jinlan;ZENG Yuanhuan;LI Keke;TANG Huiwu(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510631,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第3期451-459,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
广东省自然科学基金项目(2021A1515010517,2022A1515010798)
大学生创新创业训练计划项目(S202211347059)。
