摘要
[背景]环境噪声污染严重,目前关于长期睡眠期噪声暴露对认知功能影响及可能的生物钟机制相关研究较少。[目的]探讨睡眠期噪声暴露诱导小鼠认知功能损伤及可能的生物钟机制,为噪声暴露防护提供理论依据。[方法]20只雄性C57BL/6J小鼠随机分为对照组和噪声暴露组,每组10只。噪声暴露组采用噪声发生器进行每天12 h(08∶00—20∶00),共30 d的睡眠期噪声暴露,校准标定噪声强度为90 dB。对照组不施加任何干预措施。噪声暴露结束后,采用新物体识别实验和旷场实验检测小鼠认知功能,对小鼠海马组织进行尼氏染色,对离子钙结合适配器分子1蛋白(Iba-1)进行免疫荧光化学染色,采用实时荧光定量PCR检测炎症因子、生物钟基因表达,并采用试剂盒检测小鼠海马组织氧化应激指标变化。[结果]睡眠期噪声暴露后,新物体识别实验结果显示:与对照组相比,噪声暴露组小鼠辨别指数为0.06±0.04,低于对照组的0.65±0.13(P<0.05)。旷场实验结果显示:噪声暴露组中心活动距离为(242.20±176.10)mm,低于对照组的(1548.00±790.30)mm(P<0.05);噪声暴露组中心活动时间为(0.87±0.64)s,低于对照组的(6.00±2.86)s(P<0.05)。尼氏染色结果显示:与对照组相比,噪声暴露组小鼠海马组织神经元固缩,染色加深,排列紊乱,连接松散。免疫荧光结果显示:与对照组相比,噪声暴露组小鼠海马组织小胶质细胞活化,Iba-1表达增加(P<0.05)。实时荧光定量PCR结果显示:与对照组相比,噪声暴露组小鼠生物钟基因Clock、Per2、Rev-erbαmRNA水平增加(P<0.05),Per1 mRNA水平降低(P<0.05),海马组织IL-18、IL-6、i NOS、NLRP3 mRNA水平增加(P<0.05)。氧化应激指标检测结果显示:与对照组相比,噪声暴露组还原型谷胱甘肽含量降低(P<0.001)。[结论]睡眠期噪声暴露可导致海马组织生物钟基因失稳态并引发海马神经炎症,促使小胶质细胞激活,引起小鼠的认知功能�
[Background]Environmental noise pollution is serious,and there are few studies on the effects of long-term noise exposure during sleep on cognitive function and possible biological clock mechanism.[Objective]To explore the cognitive impairment induced by noise exposure during sleep in mice and possible biological clock mechanism,and to provide a theoretical basis for the protection against noise exposure.[Methods]Twenty male C57BL/6J mice were randomly divided into a control group and a noiseexposed group,10 mice in each group.The noise-exposed group was exposed to sleep-period noise using a noise generator for 12 h(08:00–20:00)per day for a total of 30 d.The calibrated noise intensity was set at 90 dB.No intervention was imposed on the control group.At the end of the noise exposure,cognitive function of mice was examined using the new object recognition experiment and the open field test,and the hippocampal tissue damage of mice were evaluated by Nissl staining,ionized calcium binding adaptor molecule 1(Iba1)immunofluorescence staining,and real-time fluorescence quantitative PCR for inflammatory factors and biological clock genes.Oxidative stress indicators in the hippocampus of mice were also detected by assay kit.[Results]After noise exposure during sleep period,the results of new object recognition experiment showed that the discrimination index of mice in the noise-exposed group was 0.06±0.04,which was significantly lower than that of the control group(0.65±0.13)(P<0.05).The results of open field test showed that the central activity distance of the noise-exposed group was(242.20±176.10)mm,which was significantly lower than that of the control group,(1548.00±790.30)mm(P<0.05),and the central activity time of the noise-exposed group was(0.87±0.64)s,which was significantly lower than that of the control group,(6.00±2.86)s(P<0.05).The Nissl staining results showed that compared with the control group,neurons in the hippocampus of the noise-exposed mice were shrunken,deeply stained,disorganized,and loose
作者
付一鸣
张鑫垚
佘晓俊
朱英文
杨红莲
杲修杰
付波
崔博
FU Yiming;ZHANG Xinyao;SHE Xiaojun;ZHU Yingwen;YANG Honglian;GAO Xiujie;FU Bo;CUI Bo(Institute of Environmental and Operational Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Tianjin 300050,China;Division 42,Unit 92619 of the People's Liberation Army,Sanya,Hainan 572001,China)
出处
《环境与职业医学》
CAS
CSCD
北大核心
2024年第2期119-124,132,共7页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金面上项目(81673136)。
关键词
睡眠期噪声暴露
神经炎症
认知损伤
生物钟
sleep phase noise exposure
neuroinflammation
cognitive impairment
biological clock
