摘要
目的 探讨推拿[扌衮]法对家兔骨骼肌钝挫伤后相关纤维蛋白表达的影响,以及对骨骼肌钝挫伤修复的作用机制。方法 健康成年新西兰兔15只,随机分为空白组(A)、模型组(B)和治疗(C)组,每组5只。使用自制改良重力锤打击装置对模型组和治疗组建立骨骼肌钝挫伤模型,治疗组家兔于造模成功后7 d给予推拿[扌衮]法干预,频率140次/min,2次/d,3 min/次,共治疗3 d。干预结束后1 d进行取材。HE和Masson染色观察兔股四头肌病理改变;Western-blot检测各组股四头肌基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)、特异性抑制剂-1(tissue inhibitor of metalloproteinases-1,TIMP-1)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)及I型胶原蛋白(I collagen,COL-I)表达。结果 空白组肌组织结构规整;模型组肌组织形态迥异,边界模糊,间隙显著增宽,炎细胞浸润显著,四周胶原纤维生成增加;治疗组肌组织结构相对完整,间隙缩小,可见少量炎细胞浸润与胶原纤维生成,其病理修复程度显著优于模型组。检测家兔股四头肌MMP-9、TIMP-1、TGF-β1、COL-I表达,与空白组相比,模型组与治疗组明显增加(P<0.01);但治疗组显著低于模型组(P<0.01)。结论 推拿[扌衮]法能够通过减少细胞外基质过度沉积抑制骨骼肌纤维化,其作用机制可能与下调MMP-9、TIMP-1、TGF-β1及COL-I表达量有关。
Objective To investigate the effect of tui na rolling method on the expression of relevant fibrosis proteins after blunt contusion injury of skeletal muscle in rabbits,and to explore the mechanism of action of tui na rolling method on the repair of blunt contusion injury of skeletal muscle.Methods Fifteen healthy adult New Zealand rabbits were randomly divided into three groups of blank(A),model(B) and treatment(C),with five rabbits in each group.Blunt contusion of skeletal muscle was modelled in rabbits of groups B and C using a self-constructed modified gravity hammer percussion device.The rabbits in group C were given the intervention 7 d of tui na rolling after successful modelling,at a frequency of 140 beats/min,2times/d,3 min/session,for a total of 3 d of treatment.Tissue sampling was performed 1 d after the end of the intervention.Histological changes of rabbit quadriceps femoris muscle were observed by HE and Masson staining,and MMP-9,TIMP-1,TGF-β1,and COL-I expression of quadriceps femoris muscle of rabbits in each group were detected by Western-Blot.Results The muscle tissue of the blank group was of regular structure,the muscle tissue of the model group was of very distinct shape,with blurred margins,significantly enlarged gaps,significant invasion of inflammatory cells,and increased collagen fiber production in the surrounding area,the muscle tissue of the treatment group was relatively more intact in structure,with the gaps narrowed,and a small amount of invasion of inflammatory cells and collagen fiber production could be seen,and the degree of its pathological repair was significantly better than that of the model group.Compared with the blank group,the expression of MMP-9,TIMP-1,TGF-β1,and COL-I in quadriceps femoris muscle of rabbits in the model group and the treatment group were significantly increased(P<0.01).However,the expression of MMP-9,TIMP-1,TGF-β1,and COL-I in quadriceps femoris muscle of rabbits in the treatment group was significantly lower than that in the model group(P<0.01).Conc
作者
阮磊
黄博
王兰兰
薛惠天
孙梦龙
段苗苗
彭亮
Ruan Lei;Huang Bo;Wang Lanlan;Xue Huitian;Sun Menglong;Duan Miaomiao;Peng Liang(College of Acupuncture,Massage and Rehabilitation,Hunan University of Traditional Chinese Medicine,Changsha 410208,Hunan Province,China)
出处
《中国临床解剖学杂志》
CSCD
北大核心
2024年第2期191-195,共5页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金面上项目(82174521)。
