摘要
研究了水环境中聚苯乙烯(PS)纳米塑料的共存对应急杀藻剂CuSO_(4)抑制铜绿微囊藻的影响作用。带不同基团3种聚苯乙烯纳米塑料单独处理和CuSO_(4)+纳米塑料共存情况下,通过测定藻密度、叶绿素a(Chl a)含量、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性,研究了聚苯乙烯纳米塑料与Cu^(2+)对铜绿微囊藻生长抑制的作用机制。结果表明,PS-COOH、PS、PS-NH_(2)这3种纳米塑料均能缓解CuSO_(4)对藻细胞胁迫。与空白对照组(CK)相比,CuSO_(4)、PS-COOH+CuSO_(4),PS+CuSO_(4)和PS-NH^(2+)CuSO_(4)暴露后藻密度分别抑制了42%、7%、5%、36%,Chl a含量降低了55%、6%、7%和45%,说明PS-NH_(2)与CuSO_(4)共同暴露对藻细胞生长和叶绿素合成的抑制作用与其他2种纳米塑料更为显著。相应地,PS-COOH、PS这2种纳米塑料显著缓解了CuSO_(4)对藻细胞内MDA含量、SOD活性的胁迫。与空白对照相比,PS-COOH+CuSO_(4)和PS+CuSO_(4)处理组MDA含量和SOD活性分别增加了31%、35%和7%、5%,而CuSO_(4)和PS-NH^(2+)CuSO_(4)处理后MDA含量和SOD活性分别增加了99%、66%和22%、5%。同样的,除了PS-NH_(2)外,其他2种纳米塑料均能显著降低在CuSO_(4)处理蓝藻水华过程中铜绿微囊藻胞外藻毒素(MCs)的释放。以上结果表明,带不同基团PS纳米塑料的共存在不同程度上影响CuSO_(4)的除藻效率。
In this study,we explored the effects of CuSO_(4) on a toxigenic strain of Cyanobacterium(Microcystis aeruginosa)in the presence of polystyrene nanoplastics(PS NPs).We investigated the individual impact of three different functionalized PS NPs(PS-COOH,PS,PS-NH_(2)),CuSO_(4),and their combined effects on cell density,chlorophyll a(Chl a),malondialdehyde(MDA)content and superoxide dismutase(SOD)activity.The results demonstrated that the combination of PS NPs alleviated the toxicity of CuSO_(4) to Cyanobacterium.Compared with the blank control(CK),after exposure to CuSO_(4),PS-COOH+CuSO_(4),PS+CuSO_(4) and PS-NH^(2+)CuSO_(4),the cell density of Cyanobacterium was inhibited by 42%,7%,5%and 36%,respectively,and the Chl a content was decreased by 55%and 6%,7%and 45%.The results indicated that the co-exposure of PS-NH_(2) and CuSO_(4) had more significant inhibitory effects on Cyanobacterium growth and chlorophyll synthesis than the other two PS NPs.Accordingly,PS-COOH and PS significantly alleviated the toxic stress of CuSO_(4) on the activities of MDA and SOD in Cyanobacterium.Compared with blank control,the MDA content and SOD activity in Cyanobacterium exposed to PS-COOH+CuSO_(4) and PS+CuSO_(4) treatment groups were increased by 31%and 35%,as well as 7%and 5%,respectively.While MDA content and SOD activity in Cyanobacterium exposed in CuSO_(4) and PS-NH^(2+)CuSO_(4) treatment groups were increased by 99%and 66%,as well as 22%and 5%,respectively.Similarly,except for PS-NH_(2),the other two PS NPs can significantly reduce the extracellular microcystins(MCs)release from Microcystis aeruginosa during CuSO_(4) treatment.Our findings illustrate the importance of taking the nanoplastics into account before C uSO_(4) is applied to control cyanobacteria bloom.
作者
希日古丽·麦木提敏
土玛日斯·木合塔尔
王云
努扎艾提·艾比布
Xirigul Mamtimin;Tumaris Muhtar;Wang Yun;Nuzahat Habibul(Institute of Chemistry and Chemical Technology,Xinjiang Normal University,Urumqi 830054,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2024年第2期359-366,共8页
Asian Journal of Ecotoxicology
基金
国家级大学生创新项目(202210762002)
新疆天山英才-青年拔尖人才计划项目(2022TSYCCX0010)。
