摘要
目的基于网络药理学分析麻射安金丸治疗慢性阻塞性肺疾病(Chronic obstructive pulmonary disease,COPD)的潜在机制。方法应用TCMSP中药系统药理学数据库及相关文献资料等检索麻射安金丸中的生物活性成分,筛选出作用靶点,构建麻射安金丸的药物-有效成分-作用靶点网络。根据Drugbank和Genecards等数据库建立其与COPD的相关疾病靶点,并与麻射安金丸的有效成分靶点相匹配获得潜在治疗靶点。基于Metascape数据库和DAVID数据库对潜在治疗靶点进行GO生物过程、KEGG信号通路分析,并分析蛋白质-蛋白质相互作用(Protein-protein interaction,PPI),用Cytoscape软件对PPI网络进行拓扑分析。结果麻射安金丸中有效化学成分有1525种,包括半夏22种、赤芍18种、川芎22种、甘草95种、僵蚕3种、苦杏仁26种、麻黄43种、牡蛎1种、木香29种、砂仁23种、射干12种和浙贝母5种,有效成分作用靶点基因298个。在药物-有效成分-靶点网络内重要性前10的有效成分中,来源自麻黄6种,赤芍、苦杏仁、僵蚕3种,射干、甘草、半夏、川芎、砂仁2种,浙贝母1种。KEGG富集分析显示,在得出的全部20调信号通路中,得到10条靠前的信号通路。GO功能富集分析显示,前10个生物过程包括酶结合、DNA依赖的转录正调控等;前10个分子功能包括核质、细胞质等;前10个细胞定位包括蛋白质变性调节、蛋白质代谢调节等。PPI网络经提取后获得了包含21个节点的核心网络,其中JUN、MAPK1、MAPK3、TNF、RELA、MYC、STAT3、TP53、AKT1、MAPK8、EGFR、MAPK14和PRKCA基因是核心靶点。动物实验显示:模型组和对照组大鼠血清IL-1β、IL-6和TNF-α均高于空白组,差异有统计学意义(P<0.05);模型组大鼠血清IL-1β、IL-6和TNF-α均低于对照组,差异有统计学意义(P<0.05)。模型组和对照组大鼠MAPK1、MAPK3、MYC的mRNA表达水平均高于空白组,差异有统计学意义(P<0.05);模型组大鼠MAPK1�
Objective To explore the potential mechanism of Mashe Anjin Pills in the treatment of chronic obstructive pulmonary disease(COPD)based on network pharmacology.Methods The active components and corresponding targets of Mashe Anjin Pills were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and related articles,on the basis of which the drug-active component-target network of Mashe Anjin Pills was established.The targets related to COPD were retrieved from Drugbank and Genecards,and the common targets shared by COPD and the active components of Mashe Anjin Pills were selected as the potential treatment targets.Metascape and DAVID were used for GO and KEGG pathway enrichment of the treatment targets,and the protein-protein interaction(PPI)network was established.Cytoscape was employed to analyze the topology of the PPI network.Results A total of 1525 active components in Mashe Anjin Pills were retrieved,including 22 in Pinelliae Rhizoma,18 in Paeoniaeradix Rubra,22 in Chuanxiong Rhizoma,95 in Glycyrrhizae Radix et Rhizoma,3 in Bombyx Batryticatus,26 in Armeniacae Semen Amarum,43 in Ephedrae Herba,1 in Ostreae Concha,29 in Aucklandiae Radix,23 in Amomi Fructus,12 in Belamcandae Rhizoma,and 5 in Fritillariae Thunbergii Bulbus,which corresponded to 298 targets.Among the top 10 important active components in the drug-active component-target network,6 were from Ephedrae Herba,3 from Paeoniaeradix Rubra,Bombyx Batryticatus,and Armeniacae Semen Amarum,2 from Belamcandae Rhizoma,Glycyrrhizae Radix et Rhizoma,Pinelliae Rhizoma,Chuanxiong Rhizoma,and Amomi Fructus,and 1 from Fritillariae Thunbergii Bulbus.KEGG enrichment analysis revealed the top 10 of the 20 modulated signal pathways.GO enrichment results showed that the top 10 biological processes included enzyme binding and DNA-dependent positive transcriptional regulation;the top 10 cellular components included cytoplasm and cytoplasm;the top 10 molecular functions included protein denaturation regulation and protei
作者
尚艳花
邓敬华
张晓阳
马真
王玉栋
SHANG Yan-hua;DENG Jing-hua;ZHANG Xiao-yang;MA Zhen;WANG Yu-dong(Hengshui Hospital of Traditional Chinese Medicine,Hengshui Hebei 053000)
出处
《世界中西医结合杂志》
2024年第3期542-549,共8页
World Journal of Integrated Traditional and Western Medicine
基金
河北省中医药管理局中医药科研项计划课题(2022274)。
关键词
麻射安金丸
慢性阻塞性肺疾病
网络药理学
基因
核心靶点
Mashe Anjin Pills
Chronic Obstructive Pulmonary Disease
Network Pharmacology
Gene
Core Target
