摘要
目的研究CMS患者外周血红细胞表面免疫分子CD35、CD44、CD58、CD59表达变化。方法选取自2019年11月-2021年1月就诊于青海大学附属医院的CMS患者设为CMS组,另选取在青海大学附属医院体检中心的健康人(血象正常)设为正常对照组。两组研究对象均为长期居住于海拔2500~4500 m的汉族男性,各20例。采用流式细胞术检测CMS患者外周血的成熟红细胞表面CD35、CD44、CD58、CD59表达变化,同时分离部分红细胞,测定其对肿瘤细胞的快速天然免疫黏附反应,比较两组临床资料。结果CMS组患者外周血红细胞表面CD35、CD44表达阳性率低于正常对照组,差异有统计学意义(P<0.05),CD58、CD59表达比较,差异无统计学意义(P>0.05)。CMS组患者肿瘤红细胞花环率低于正常对照组,差异有统计学意义(P<0.05);其中5例长期居住在平均海拔4500 m的CMS组患者外周血红细胞表面CD44、CD35、CD58、CD59表达阳性率均低于15名长期居住在平均海拔3250 m的CMS患者,但差异无统计学意义(P>0.05)。结论慢性高原病患者的红细胞免疫功能减低可能与CMS患者抵抗力下降及感染的发生相关。
Objective To study the expression of immune molecules CD35,CD44,CD58 and CD59 on the surface of red blood cells in peripheral blood of patients with CMS.Methods CMS patients who were admitted to the Affiliated Hospital of Qinghai University from November 2019 to January 2021 were selected as CMS group,and healthy people(normal blood picture)in the physical examination center of the Affiliated Hospital of Qinghai University were selected as normal control group.The subjects of the two groups were Han men who lived at an altitude of 2500-4500 m for a long time,with 20 men in each group.Flow cytometry was used to detect the expression of CD35,CD44,CD58 and CD59 on the surface of mature red blood cells in peripheral blood of CMS patients.At the same time,some red blood cells were isolated to determine their rapid natural immune adhesion to tumor cells.The clinical data of the two groups were compared.Results The positive expression rates of CD35 and CD44 on the surface of peripheral blood red blood cells in the CMS group were lower than those in the normal control group,and the difference was statistically significant(P<0.05).The tumor erythrocyte rosette rate in the CMS group was lower than that in the normal control group,and the difference was statistically significant(P<0.05).The positive expression rates of CD44,CD35,CD58 and CD59 on the surface of peripheral blood red blood cells in 5 CMS patients who lived at an average altitude of 4500 m for a long time were lower than those in 15 CMS patients who lived at an average altitude of 3250 m for a long time,but the difference was not statistically significant(P>0.05).Conclusion The decrease of erythrocyte immune function in patients with chronic mountain sickness may be related to the decrease of resistance and the occurrence of infection.
作者
唐梦
朱黎明
马轶
朱明明
TANG Meng;ZHU Li-ming;MA Yi;ZHU Ming-ming(Department of Hematology,the Affiliated Hospital of Qinghai University,Xining 810000,Qinghai,China;Nursing College of Anhui University of Chinese Medicine,Hefei 230000,Anhui,China)
出处
《医学信息》
2024年第7期83-88,共6页
Journal of Medical Information
基金
青海省科技计划(编号:2021-ZJ-966Q)
青海大学2019年度中青年科研基金项目(编号:2019-QYY-5)。
