摘要
目的:鉴定导致铜陵白姜茎基腐病的病原菌科贝肠杆菌(Enterobacter kobei)及其相应的噬菌体,为防治铜陵白姜茎基腐提供科学依据。方法:选用发病的铜陵白姜姜块组织作为试验材料,使用TTC和LB培养基从铜陵白姜腐烂的姜块中分离出科贝肠杆菌SJ16菌株,同时通过半固体培养基从根际土壤中分离得到噬菌体KB-P2。使用2种感染方法(根茎接种法和姜切片接种法),评定该菌株对铜陵白姜的致病性;并利用注射法测定噬菌体的防控效果。结果:共分离出103株细菌分离株,经LB培养基筛选得到10株菌株,其中SJ16菌株经鉴定为科贝肠杆菌(E.kobei)。病原侵染试验表明,该菌株是导致铜陵白姜枯萎的致病菌,并会在后期引起铜陵白姜根茎腐烂。此外,分离纯化得到3种科贝肠杆菌噬菌体,通过双层平板培养方法筛选出裂解能力最强的KB-P2噬菌体。同时,噬菌体接种试验也进一步显示,KB-P2噬菌体具有良好的防控效果。结论:科贝肠杆菌SJ16菌株是一种导致铜陵白姜茎基腐的新病原菌,而KB-P2噬菌体能够有效降低铜陵白姜茎基腐的发病率。该研究为进一步探讨铜陵白姜茎基腐的防治提供了重要依据。
Objective:To identify the pathogen(Enterobacter kobei)of the stem base rot of Tongling white ginger and its corresponding bacteriophage,and to provide scientific basis for the prevention and control of stem base rot of Tongling white ginger.Methods:E.kobei SJ16 was isolated from diseased tissues of Tongling white ginger using both TTC and LB medium,and bacteriophage KB-P2 was isolated from rhizosphere soil using semi-solid medium.The pathogenicity of this strain to Tongling white ginger was evaluated by two infection methods(rhizome inoculation and ginger slice inoculation).The control effect of bacteriophage was measured using the injection method.Results:A total of 103 bacterial isolates were isolated and 10 strains were obtained by LB medium screening,among which SJ16 was identified as E.kobei.Infection experiment showed that the strain was the pathogen that caused the blight of Tongling white ginger,and could cause root rot in the later stage.In addition,three bacteriophages of E.kobei were isolated and purified,and KB-P2 bacteriophage with the strongest cleavage ability was screened by double-layer plate culture method.The infection experiment showed that the bacteriophage KB-P2 had greater control effect.Conclusion:SJ16 was one new pathogen causing stem base rot of Tongling white ginger,while KB-P2 could effectively reduce the incidence of stem rot of Tongling white ginger.Therefore,this study provided an important basis for further research on the prevention and control of stem rot of Tongling white ginger.
作者
花月
汪菁森
周成
HUA Yue;WANG Jingsen;ZHOU Cheng(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China;College of Life and Health Sciences,Anhui Science and Technology University,Fengyang 233100,China;College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China)
出处
《安徽科技学院学报》
2024年第2期46-52,共7页
Journal of Anhui Science and Technology University
基金
安徽省重点研究与开发计划项目(202004a06020003)
安徽省自然科学基金面上项目(2108085MC107)
安徽省大学生创新创业训练计划项目(S202310879207)。
关键词
铜陵白姜
病原鉴定
茎基腐
科贝肠杆菌
噬菌体
Tongling white ginger
Pathogen identification
Stem rot
Enterobacter kobei
Bacteriophage
