摘要
采用微波消解法处理样品,ICP-MS和ICP测定蛇中43种无机元素含量,利用主成分分析、正交偏最小二乘判别分析和线性判别分析对乌梢蛇、酒乌梢蛇、金钱白花蛇、酒炒金钱白花蛇中无机元素含量数据进行统计分析。结果表明:Li、Cr、Mn、Ni、Zr、Y、La、Ce、Pr、Nd、Sm、Gd、Tb、Dy、Ho和Tm 16种元素在4种蛇样品中相对含量趋势基于一致,4种蛇样品中,乌梢蛇中Cu、Ti含量高,Zn含量低,酒乌梢蛇中V、As、Mo、Cd、Sb、Co、Sr、Ba含量高;金钱白花蛇中Lu含量高,酒炒金钱白花蛇中Ga、Fe、Al含量高,不同蛇样品具有独特的无机元素含量。主成分分析提取的5个主成分,累计方差贡献率为84.38%;主成分分析和OPLS-DA可以完全区分乌梢蛇和金钱白花蛇;线性判别模型对乌梢蛇、酒乌梢蛇、金钱白花蛇、酒炒金钱白花蛇的判别正确率为100%.实验结果证明基于蛇样品中无机元素含量所建立的判别模型可以有效的识别乌梢蛇、酒乌梢蛇、金钱白花蛇、酒炒金钱白花蛇,为蛇的真伪、分类判别提供基础数据和技术支撑。
Samples were treated with microwave digestion.The contents of 43 inorganic elements in snakes were de-termined by ICP-MS and ICP.The inorganic element content data of Zaocys,Zaocys broiled by wine,Bungaruspar-vus and Bungarusparvus stirred-fried with wine were statistically analyzeed by principal component analysis,orthogo-nal partial least squares discriminant analysis,and linear discriminant analysis..The results showed that the relative content trends of 16 elements,Li,Cr,Mn,Ni,Zr,Y,La,Ce,Pr,Nd,Sm,Gd,Tb,Dy,Ho,and Tm,were consistent in the four snake samples.Among the these samples,the contents of Cu and Ti were high while the content of Zn was low in Zaocys.The contents of V,As,Mo,Cd,Sb,Co,Sr,and Ba were high in Zaocys broiled by wine.The content of Lu was high in Bungarusparvus,while the contents of Ga,Fe and Al were high in Bungarusparvus stirred-fried with wine.Different snake samples have unique inorganic element contents.The cumulative variance contribution rate of the five principal components extracted by principal component analysis is 84.38%.the principal component analysis and OPLS-DA can completely distinguish between Zaocys and Bungarusparvus.The linear discriminant model has a 100%accuracy rate for distinguishing Zaocys,Zaocys broiled by wine,Bungarusparvus and Bungarus-parvus stirred-fried with wine.The experimental results show that the discriminant model based on the content of in-organic elements in snake samples can effectively identify Zaocys,Zaocys broiled by wine,Bungarusparvus and Bun-garusparvus stirred-fried with wine,and provide basic data and technical support for the authenticity and classifica-tion of snakes.
作者
洪家顺
邹艺荣
王裕成
李道成
彭思思
Hong Jiashun;Zhou Yirong;Wang Yucheng;Li Daocheng;Peng Sisi(Jiangxi Provincial DaodiHerbal Quality Evaluation Research Center,Nanchang 330029,China)
出处
《西藏科技》
2024年第2期3-12,共10页
Xizang Science And Technology
关键词
乌梢蛇
金钱白花蛇
无机元素
判别分析
Zaocys dhumnades
Bungarus Parvus
Inorganic elements
Discriminant analysis
