摘要
旨在为富含多不饱和脂肪酸(PUFA)的中长链脂肪酸甘油三酯(MLCT)产品的应用提供参考,采用脂肪酶Lipozyme TL IM催化精炼桑蚕蛹油(SPO)与三辛酸甘油酯(TRI)进行酯交换反应合成富含α-亚麻酸(ALA)的MLCT(ALA-MLCT),考察了反应时间对ALA-MLCT合成的影响,并对合成的ALA-MLCT的甘油三酯组成、质量指标、氧化稳定性、乳液性能和体外消化特性进行了分析。结果表明:Lipozyme TL IM催化效率高,反应10 h TRI的转化率达到98%以上,反应12 h合成的产物ALA-MLCT中含有ALA的MLCT含量可以达到40.41%,且主要为LnCaCa (10.95%)、PLnCa (7.69%)和OLnCa (6.21%),其质量指标符合中长链脂肪酸食用油标准征求意见稿2022中的一级食用油标准;与SPO和酯交换反应底物物理混合物(SPO与TRI质量比1∶4,SPO+TRI)相比,产物ALA-MLCT氧化稳定性显著提高,其乳液粒径较小且分布均匀,具有更好的物理稳定性;体外模拟消化实验表明,与SPO+TRI相比,ALA-MLCT更快达到消化平衡,但最终二者消化率均可以达到90%以上。综上,合成的ALA-MLCT结构脂有助于提高ALA的生物可及性,有望作为功能性食用油脂。
In order to provide reference for the application of medium-and long-chain fatty acid triglycerides(MLCT)products enriched with polyunsaturated fatty acids(PUFA),lipase Lipozyme TL IM was employed to catalyze the transesterification of refined silkworm pupa oil(SPO)and tricaprylin(TRI)to synthesize MLCT rich inα-linolenic acid(ALA)(ALA-MLCT).The impact of reaction time on the synthesis of ALA-MLCT was studied,and the triglycerides composition,quality index,oxidative stability,emulsion performance,and in vitro digestion characteristics of the ALA-MLCT were analyzed.The results showed that Lipozyme TL IM had high catalytic efficiency,and the conversion rate of TRI reached more than 98% after 10 h of reaction. The content of MLCTcontaining ALA in ALA - MLCT synthesized by transesterification reaction for 12 h reached 40. 41%,and it was mainly composed of LnCaCa ( 10. 95% ) ,PLnCa ( 7. 69%) and OLnCa ( 6. 21%) . Thequality indexes of ALA -MLCT complied with the standards of first - class edible oil in the medium - andlong - chain fatty acid edible oil standard draft 2022. Compared with SPO and the physical mixture oftransesterification reaction substrate ( SPO + TRI,in a 1 ∶ 4 mass ratio ) ,ALA - MLCT exhibitedsignificantly improved oxidation stability,and its emulsion had better physical stability,with smaller andmore evenly distributed particles size. In vitro simulated digestion experiments revealed that ALA - MLCTachieved a faster digestive balance compared with SPO + TRI,but the final digestibility of both couldexceed 90%. In conclusion,the synthesized ALA - MLCT can help to improve the bioaccessibility ofALA and is expected to be used as functional edible oil.
作者
陈福妮
刘琛
王卫飞
钟赛意
王思远
穆利霞
廖森泰
邹宇晓
CHEN Funi;LIU Chen;WANG Weifei;ZHONG Saiyi;WANG Siyuan;MU Lixia;LIAO Sentai;ZOU Yuxiao(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,Guangdong,China;Guangdong key Laboratory of Agricultural Products Processing,Key Laboratory of Functional Food,Ministry of Agriculture and Rural Affairs,Sericultural&Agri-Food Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2024年第3期66-71,共6页
China Oils and Fats
基金
财政部和农业农村部国家现代农业产业技术体系资助(CARS-18-ZJ0503)
佛山市财政专项资金——高水平广东省农业科技示范市建设资金市院合作项目
广东省农业科学院地方分院和专家工作站工作经费项目(2023支撑01)。
关键词
中长链脂肪酸结构脂
Α-亚麻酸
酶法合成
氧化稳定性
medium-and long-chain triacylglycerols
α-linolenic acid
enzymatic synthesis
oxidative stability