摘要
采用一种绿色简单的电化学方法将还原氧化石墨烯-金纳米复合薄膜共沉积到玻碳电极(GCE)上,作为传感器用于L-酪氨酸(L-Tyr)的检测.采用扫描电子显微镜(SEM)、循环伏安法(CV)和电化学阻抗谱(EIS)对修饰电极进行了表征.采用差分脉冲伏安法(DPV)研究了0.1 mol/L磷酸盐缓冲溶液中L-Tyr在修饰电极上的电化学行为,发现L-Tyr在修饰电极上的伏安响应比裸GCE明显提高.对复合薄膜的厚度、支撑电解质的p H值、沉积电位和积累时间进行了优化;在最佳实验条件下, L-Tyr的氧化峰电流在0.1~50μmol/L及50~1000μmol/L浓度范围内呈现良好的线性关系.该传感器的检出限为50 nmol/L,灵敏度为0.553μA·μmol·L^(-1),具有良好的重复性、稳定性和抗干扰性.
Using a green and simple electrochemical method,a reduced graphene oxide-gold nanocomposite thin film was prepared for the detection of L-tyrosine(L-Tyr).The modified electrode was characterized via scanning electron microscopy(SEM),cyclic voltammetry(CV),and electrochemical impedance spectroscopy(EIS).The electrochemical behavior of L-Tyr on the modified electrode in 0.1 mol/L phosphate buffer solution was investigated using differential pulse voltammetry(DPV).The results demonstrated a significantly enhanced voltammetric response of L-Tyr on the modified electrode compared to a bare glassy carbon electrode(GCE).Optimization of the composite film’s thickness,pH of the supporting electrolyte,deposition potential,and accumulation time were carried out.Under the optimal experimental conditions,the oxidation peak current of L-Tyr exhibited a good linear relationship within the concentration ranges of 0.1—50μmol/L and 50—1000μmol/L using DPV.Moreover,the sensor demon⁃strated a detection limit of 50 nmol/L,a sensitivity of 0.553μA·μmol·L^(-1),and displayed excellent repeatability,stability,and anti-interference properties.
作者
马勤政
王伟
梁旭婷
MA Qinzheng;WANG Wei;LIANG Xuting(Hebei University of Technology,School of Electronics and Information Engineering,Tianjin 300401,China;Tianjin Key Laboratory of Electronic Materials and Device,Tianjin 300401,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2024年第4期10-18,共9页
Chemical Journal of Chinese Universities
基金
河北省自然科学基金(批准号:F2019202377)资助。
