摘要
目的探究激活素A受体Ⅰ型(ACVR1)在胃癌中的表达、预后预测价值及临床意义。方法通过癌症基因组图谱(TCGA)数据库下载375例胃癌转录组数据、32例正常胃组织转录组数据及临床相关参数, 利用Wilcoxon秩和检验分析ACVR1 mRNA的表达差异, 采用卡方检验分析ACVR1表达量与临床病理特征的相关性。通过基因表达交互网站(GEPIA)及单多因素回归分析胃癌患者预后影响因素。其中, 采用Cox回归模型进行单因素和多因素分析。同时, 通过R语言分析ACVR1基因的相关通路, 探索其在胃癌中的潜在机制。结果 ACVR1在胃癌组织中的mRNA表达量显著高于正常组织[4.673(4.247, 5.087)比3.699(3.332, 4.426), Z=16.6, P<0.01], 其表达量与肿瘤浸润深度呈正相关(χ^(2)=11.521, P<0.01);TCGA数据库分析显示胃癌组织中ACVR1高表达患者预后差(LogrankP<0.01);多因素分析结果显示ACVR1表达(风险比=1.639, 95%可信区间:1.102~2.436, P<0.05)是影响胃癌预后的独立因素;京都基因与基因组百科全书(KEGG)通路富集分析显示ACVR1参与磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(Akt)信号通路、神经活性配体-受体相互作用、黏着斑、钙信号、人类乳头状瘤病毒感染等相关通路。结论 ACVR1 mRNA在胃癌组织中高表达, 且与胃癌患者的不良预后密切相关, 其可能通过PI3K-Akt信号通路、神经活性配体-受体相互作用、黏着斑通路等在胃癌中发挥作用。
Objective To explore the expression,prognostic value and clinical significance of activin A receptor typeⅠ(ACVR1)in gastric cancer(GC).Methods The transcriptome data and clinical parameter of GC were downloaded through the Cancer Genome Atlas(TCGA)database.The mRNA expression of ACVR1 and the its correlation with clinicopathological features were analyzed by R.The prognostic factors of GC patients were explored by the gene expression profiling interactive analysis(GEPIA)database and Multivariate analysis.At the same time,the relevant pathways of ACVR1 were analyzed by R in order to explore its potential mechanism in GC.Results The mRNA expression of ACVR1 in GC was significantly higher[4.673(4.247,5.087)vs.3.699(3.332,4.426),Z=16.6,P<0.01],and its expression was significantly correlated with the tumor invasion depth(χ^(2)=11.521,P<0.01).TCGA database analysis showed that the overall survival time/progression-free survival time in GC patients with high ACVR1 expression were shorter than in GC patients with low ACVR1 expression(P<0.01).Moreover,multivariate analysis showed that ACVR1 expression[hazard ratio=1.639,95%confidence interval:1.102-2.436,P<0.05]was an independent predictor of the prognosis of GC patients.The kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis showed that ACVR1-related differential genes were mainly enriched in the phosphatidylinositol 3 kinase(PI3K)-protein kinase B(Akt)signaling pathway,neuroactive ligand receptor interaction signaling pathway,focal adhesion signaling pathway,calcium signaling pathway,human papillomavirus infection pathway and other related pathways.Conclusion ACVR1 mRNA is highly expressed in GC and closely related to the poor prognosis of GC patients.Moreover,it may play an important role in GC through the PI3K-Akt signaling pathway,neuroactive ligand receptor interaction signaling pathway,focal adhesion signaling pathway.
作者
吴梦婕
牛雪瑶
张鹏
王居峰
Wu Mengjie;Niu Xueyao;Zhang Peng;Wang Jufeng(Department of Internal Medicine,the Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer Hospital,Zhengzhou 450008,China;Henan Vocational College of Nursing,Anyang 455000,China;Department of Oncology,Yongcheng People’s Hospital,Yongcheng 476699,China)
出处
《中华实验外科杂志》
CAS
2024年第2期226-229,共4页
Chinese Journal of Experimental Surgery
基金
河南省科技攻关项目(212102310198)。
关键词
激活素A受体
胃癌
基因表达
预后
Activin A receptor
Gastric cancer
Gene expression
Prognosis
