摘要
[目的]探究根瘤内生菌群对南洋楹苗木的促生效应,并揭示根瘤优势内生菌组成及其与苗木生长的关系,为南洋楹菌肥研发提供参考。[方法]以来源于广东广州、惠州和茂名等3个地区的南洋楹根瘤提取液为接种菌液(YZ、YD和YX),开展南洋楹组培苗接种对比试验,并对回接根瘤进行16S rRNA基因测序鉴定和数据分析。[结果]与对照相比,3种接种处理的南洋楹苗木均能成功接根瘤,其苗期生长与结瘤、生物量积累、光合及固氮等相关指标均显著优于对照,其中苗高、根长、相对叶绿素含量和总氮量等表征南洋楹苗木的综合情况的指标分别比对照增加了53.74%~95.65%、52.91%~98.41%、74.04%~86.18%和7.6~15.9倍。3种接种处理中,接种菌液YZ对苗木促生、结瘤和固氮效应最为显著。回接根瘤内生菌群组成存在一定差异,YZ处理的根瘤内生菌的比例最均衡、细菌物种数(OTU数)最多和α多样性(香农指数)最高。根瘤内生菌中一种反硝化Fe-Ⅱ氧化细菌与南洋楹苗高、回接根瘤数呈极显著正相关,与含氮量、总氮量和固氮量呈显著正相关;OTU数和香农指数与南洋楹苗高、根瘤数、总氮量和固氮量等呈极显著或显著正相关关系。[结论]南洋楹根瘤提取液能对南洋楹苗期的生长与结瘤、生物量积累、光合及固氮起到积极的促进作用,不同来源的接种菌液对苗木的促生效应及回接根瘤内生菌群组成存在一定差异,根瘤内生菌种类越丰富、相对丰度越均衡,对南洋楹幼苗促生和固氮作用越显著。
[Objective]To reveal the composition of dominant nodule endophytes in Falcataria falcata and explore their effect on the growth of Falcataria falcata seedlings,thus providing information for the development of Falcataria falcata fertilizer.[Methods]Endophytic bacteria were screened from Falcataria falcata nodules collected from three locations(Guangzhou,Huizhou and Maoming of Guangdong province),and inoculation with the three different geographical nodule endophytes(YZ,YD and YX)were conducted.The growth traits of Falcataria falcata seedlings after inoculation with different geographical endophytic bacteria were measured.Furthermore,species identification of endophytic bacteria with different treatments was performed by using 16S rRNA gene sequencing technology.[Results]Compared with the CK(negative control),Falcataria falcata seedlings under the three treatments were able to develop nodules successfully,and the seedling growth and nodule number,biomass,photosynthesis and nitrogen fixation ability were significantly higher than those under the control treatment.Particularly,the seedling height,root length,SPAD in the three treatments increased by 53.74%~95.65%,52.91%~98.41%,74.04%~86.18%,and 7.6~15.9 times compared to the control,respectively.Among the three inoculation treatments,seedling after inoculation with YZ showed the highest values of seedling growth,nodulation number and nitrogen fixation.Significant differences in the composition of endophytic bacteria were found among the three treatments,and YZ treatment had the largest number of OTU number and the highest diversity of α(Shannon index).The denitrifying Fe-Ⅱ oxidizing bacterium had significant effects on seedlings height,nodules number,nitrogen concentration,nitrogen content and nitrogen fixation in Falcataria falcata.[Conclusion]The endophytes isolated from Falcataria falcata nodule can promote seedling growth,biomass accumulation,photosynthesis ability and nitrogen fixation in Falcataria falcata seedlings.Different geographical nodule endophytes
作者
林千禧
郑会全
路国辉
黄荣
韦如萍
王润辉
晏姝
LIN Qian-xi;ZHENG Hui-quan;LU Guo-hui;HUANG Rong;WEI Ru-ping;WANG Run-hui;YAN Shu(College of Life Sciences,South China Normal University,Guangzhou,510631,Guangdong,China;Guangdong Academy of Forestry/Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520,Guangdong,China)
出处
《林业科学研究》
CSCD
北大核心
2024年第2期134-143,共10页
Forest Research
基金
广东省林业科技创新项目(2022KJCX007)
广东省省级财政事业发展性支出项目(粤林财2022-8号)。
关键词
南洋楹
根瘤
接种菌液
内生菌群
促生效应
相关性
Falcataria falcata
Nodules
Inoculation solution
Endophytes flora
Grow-promoting effect
Correlation
