摘要
目的探讨肠易激综合征患者血清可溶性髓系细胞触发受体-1(sTREM-1)、白细胞介素-33(IL-33)的表达水平及临床意义。方法纳入2017年1月至2022年12月该院收治的103例肠易激综合征患者作为观察组,根据肠易激综合征症状严重程度量表(IBS-SSS)分为轻度组、中度组、重度组。另将同期在该院进行体检的88例健康人作为对照组。采用酶联免疫吸附试验(ELISA)检测各组血清sTREM-1、IL-33水平。采用Pearson相关分析探讨血清sTREM-1、IL-33与肠易激综合征病情程度的关系,采用受试者工作特征(ROC)曲线评估血清sTREM-1、IL-33对肠易激综合征的诊断价值。结果观察组血清sTREM-1、IL-33水平均明显高于对照组(P<0.05)。重度组肠易激综合征患者血清sTREM-1、IL-33水平均明显高于中度组、轻度组,中度组高于轻度组,差异均有统计学意义(P<0.05)。Pearson相关分析显示,肠易激综合征患者血清sTREM-1、IL-33水平与IBS-SSS评分均呈正相关(r=0.547、0.510,均P<0.05)。ROC曲线分析显示,血清sTREM-1、IL-33诊断肠易激综合征的曲线下面积(AUC)分别为0.841、0.854;二者联合评诊断肠易激综合征的AUC为0.935,灵敏度、特异度分别为87.85%、86.49%。结论肠易激综合征患者血清sTREM-1、IL-33呈高表达,且与病情程度呈正相关,有望作为诊断肠易激综合征发生的生物标志物。
Objective To investigate the expression levels of serum soluble myeloid cell triggering receptor-1(sTREM-1)and interleukin-33(IL-33)in patients with irritable bowel syndrome and their clinical significance.Methods A total of 103 patients with irritable bowel syndrome admitted to the hospital from January 2017 to December 2022 were selected as the observation group,and divided into mild group,moderate group and severe group according to the irritable bowel syndrome symptom severity scale(IBS-SSS).In addition,88 healthy people who underwent physical examination in this hospital during the same period were selected as the control group.The levels of serum sTREM-1 and IL-33 were detected by enzyme-linked immunosorbent assay(ELISA).Pearson correlation analysis was used to investigate the relationship between serum sTREM-1 and IL-33 and the severity of irritable bowel syndrome.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of serum sTREM-1 and IL-33 in patients with irritable bowel syndrome.Results The levels of serum sTREM-1 and IL-33 in the observation group were significantly higher than those in the control group(P<0.05).Serum sTREM-1 and IL-33 levels in patients with irritable bowel syndrome in severe group were significantly higher than those in moderate and mild groups,and the moderate group was higher than that in mild group,with statistical significance(P<0.05).Pearson correlation analysis showed that serum sTREM-1 and IL-33 were positively correlated with IBS-SSS scores in patients with IBS(r=0.547,0.510,both P<0.05).ROC curve analysis showed that the area under curve(AUC)of serum sTREM-1 and IL-33 in the diagnosis of IBS was 0.841 and 0.854,respectively.The AUC of IBS was 0.935,the sensitivity and specificity were 87.85%and 86.49%respectively.Conclusion The expression levels of serum sTREM-1 and IL-33 were increased in patients with irritable bowel syndrome,and were positively correlated with the severity of the disease,which is expected to be used as biomarkers for the
作者
罗小丽
胡俊泽
王文革
张明华
马建伟
LUO Xiaoli;HU Junze;WANG Wenge;ZHANG Minghua;MA Jianwei(Department of Clinical Laboratory,Linfen Central Hospital,Linfen,Shanxi 041000,China;Department of Orthopedics,Linfen Central Hospital,Linfen,Shanxi 041000,China)
出处
《国际检验医学杂志》
CAS
2024年第7期800-803,共4页
International Journal of Laboratory Medicine
基金
山西省卫生健康委科研课题计划(2020161)。
