摘要
旨在研究植物雌激素大豆黄酮(DZ)对断奶期小鼠乳腺退化的影响。选取48只孕鼠,随机分为对照组(CON组),腹腔注射无菌PBS;大豆黄酮组(DZ组),每天每kg体重腹腔注射DZ 100 mg,连续处理1周。孕鼠正常分娩后,在哺乳期第10天强制断奶。各组分别在断奶第1、3、7天选取8只小鼠取血后处死,取所有乳腺组织,称重计算乳腺指数;ELISA法测定血液中雌二醇(E2)、催乳素(PRL)、胰岛素样生长因子(IGF-Ⅰ)的浓度;常规法制作乳腺组织切片,进行HE染色;Western blot及荧光定量PCR(RT-qPCR)分析乳腺组织内促凋亡因子Bax和抗凋亡因子Bcl-2的蛋白与基因表达水平。结果:与CON组相比,DZ组增加了断奶1 d母鼠的乳腺指数,但降低断奶3 d、7 d的乳腺指数;与CON组相比,断奶1 d时,DZ组极显著提高母鼠血清中PRL和IGF-Ⅰ浓度(P<0.01),断奶3 d时DZ组极显著提高了PRL和E2浓度(P<0.01),显著提高断奶7 d母鼠血清中E2浓度(P<0.05);HE染色结果显示,与CON组相比,DZ组显著降低断奶母鼠乳腺组织中脂肪细胞面积比(P<0.05),提高腺泡的面积比(P>0.05);与CON组相比,DZ组极显著上调断奶期乳腺组织Bcl-2蛋白的表达水平(P<0.01),极显著下调断奶1 d Bax基因的表达水平(P<0.01),断奶3 d时极显著下调Bax蛋白与基因的表达水平(P<0.01),断奶7 d时极显著上调乳腺中Bax和Bcl-2蛋白的表达水平(P<0.01),在断奶1 d和3 d,Bcl-2/Bax比值均极显著升高(P<0.01)。本研究表明,DZ处理可升高断奶母鼠血清中IGF-Ⅰ、PRL及E2浓度,上调乳腺中Bcl-2/Bax比值,抑制细胞凋亡,降低脂肪细胞所占面积比,提示DZ可能通过抑制母鼠乳腺细胞凋亡,延缓乳腺早期退化。
To investigate the effects of Daidzein(DZ)on mammary gland involution in weaned mice,48 pregnant mice were used and ran⁃domly divided into a control group(treated with sterile PBS)and a DZ group(intraperitoneal injection of 100 mg/kg),once daily,for 7 days.After normal delivery,the mice were forced to wean on the 10th day of lactation.Then,the rodents were euthanized on 1,3 and 7 days after weaning,respectively,and their blood and mammary tissues were sampled for analysis.All the mammary tissues were weighed to calcu⁃late the mammary index.The enzyme-linked immunosorbent kit(ELISA)was used to detect serum estradiol(E2),prolactin(PRL)and insulin-like growth factor-Ⅰ(IGF-Ⅰ)levels in the mice.The morphological changes of mammary tissue were analyzed with HE staining.Western blotting was used to detect Bax and Bcl-2 protein expression in the breast tissue.The results showed that compared with the control group,the mammary index was increased in the DZ group on one-day weaned mice,while the index was lower than that of the control group on 3 and 7 days of weaning.Compared with the control group,on 1 day after weaning,the levels of serum PRL(P<0.01)and IGF-Ⅰ(P<0.01)were significantly increased in the DZ group.Three days after weaning,the serum PRL and E2 concentrations in the DZ group were significantly increased(P<0.01).The HE staining results showed that,compared with the control group,the adipocyte area ratio in the breast tissue of the weaned female mice in the DZ group was significantly decreased(P<0.05),and the acinar area ratio was increased(P>0.05).Compared with the control group,the expression of Bcl-2 was significantly up-regulated by DZ(P<0.01),while Bax expression was significantly decreased on 3 days after weaning(P<0.01),and the expression of Bax and Bcl-2 proteins was significantly increased on 7 days after weaning(P<0.01).The ratio of Bcl-2/Bax was significantly increased on 1 day and 3 days after weaning(P<0.01)in the DZ group,but decreased on 7 days of weaning(P>0.05).These results indicated
作者
黄心河
张亚峰
伍钢
陈雨涛
周潇
张源淑
韩正康
HUANG Xinhe;ZHANG Yafeng;WU Gang;CHEN Yutao;ZHOU Xiao;ZHANG Yuanshu;HAN Zhengkang(Key Laboratory of Animal Physiology and Biochemistry of Ministry of Agriculture and Rural Affairs/College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)
出处
《畜牧与兽医》
CAS
北大核心
2024年第4期41-48,共8页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31972640)。
关键词
植物雌激素
大豆黄酮
断奶期
乳腺退化
小鼠
phytoestrogen
Daidzein
weaning period
mammary gland involution
mice
