摘要
为明确辽宁白鲜主要病害根腐病的发生危害及病原菌的种类,本研究于2019—2020年对辽宁省白鲜主产区本溪县、清原满族自治县等8个地区进行了根腐病危害情况定点跟踪调查。采用组织分离法对不同地区采集的病样进行分离、纯化,再依据柯赫氏法则进行致病性测定并结合形态学和分子生物学鉴定。结果表明,不同白鲜种植地区病害发生率及严重程度存在一定差异,其中病害发生最高的是本溪县和清原满族自治县,病害发生率分别达7.1%~9.2%和6.1%~7.2%。经鉴定,病原菌代表菌株BX69-1与NCBI中茄病镰刀菌(Fusarium solani)的多条r DNA-ITS序列同源性达100%,因此鉴定茄病镰刀菌是引起辽宁地区白鲜根腐病的病原真菌。本研究明确了辽宁省白鲜根腐病病原菌,为白鲜生产上病害防治和抗病育种提供理论依据。
In order to identify the occurrence and damage of root rot and the types of pathogens of dictamnus(Dictamnus dasycarpus)in Liaoning Province,the study conducted a fixed-point follow-up survey of root rot dam-age in Benxi City and Qingyuan Manchu Autonomous in Liaoning Province from 2019 to 2020.The disease sam-ples collected from different areas were isolated and purified by tissue isolation method,and then the pathogenicity was determined according to Koch's law,combined with morphological and molecular biological identification.The results showed that there were some differences in the disease incidence and severity among different planting areas.The highest disease incidence was in Benxi City and Qingyuan Manchu Autonomous,with the disease inci-dence of 7.1%~9.2%and 6.1%~7.2%,respectively.The homology of the rDNA-ITS sequences between the repre-sentative strain BX69-1 and F.solani in NCBI was 100%.Therefore,F.solani was identified as the pathogen of root rot in Liaoning Province.This study provided a theoretical basis for disease control and disease resistance breeding in dictamnus production.
作者
沈宝宇
孙文松
张天静
刘坤
Shen Baoyu;Sun Wensong;Zhang Tianjing;Liu Kun(Liaoning Research Institute of Cash Crops,Liaoyang,111000;Institute of Medicinal Plants,Liaoning Academy of Agricultural Sciences,Liaoyang,111000)
出处
《分子植物育种》
CAS
北大核心
2024年第7期2265-2270,共6页
Molecular Plant Breeding
基金
财政部和农业农村部:国家现代农业产业技术体系项目
辽宁省农业科技自主创新资金项目(2020CGPY0403)
辽宁省农业科学院学科建设计划项目(2019DD144221)共同资助。
关键词
白鲜
根腐病
茄病镰刀菌
病原鉴定
BX69-1
Dictamnus dasycarpus Turcz.
Root rot
Fusarium solani
Pathogen indentification
BX69-1
