摘要
为了研究多孔材料对酶固定化性能的影响,提出了一种以地质聚合物管状无机膜为载体的新型酶固定化策略。利用该膜材料介孔中存在的大量硅羟基和铝羟基结构,采用戊二醛交联法成功地将碳酸酐酶负载到该无机介孔膜中。固定化实验结果表明:固定化酶复合膜的酶活性回收率可达63.7%。在稳定性测试中,该膜在连续使用30次后保持了67.8%的相对活性,在4℃下保存60 d后保持了52.0%的相对活性。在CO_(2)捕集试验中,CO_(2)捕集效率在固定化酶复合膜催化下提高了5.6倍。
To study the effect of porous materials on the performance of enzyme immobilization,we proposed a novel strategy for enzyme immobilization using a geopolymer tubular inorganic membrane(GM)as a carrier.Carbonic anhydrase(CA)was successfully loaded into this mesoporous membrane using glutaraldehyde(GA)cross-linking,relying on the large number of silica hydroxyl and aluminum hydroxyl structures naturally present in the membrane's mesopores.The results of the immobilization experiments show that the immobilized enzyme composite membrane(GM-CA)achieves 63.7%enzyme recovery.In stability tests,the membrane retained 67.8%relative activity after 30 consecutive uses and 52.0%relative activity after 60 days of storage at 4℃.In the CO_(2) capture assay,the CO_(2) capture efficiency developed 5.6 times under the catalytic of GM-CA.
作者
赵宝中
贺艳
邓兴发
肖培
崔学民
ZHAO Baozhong;HE Yan;DENG Xingfa;XIAO Pei;CUI Xuemin(School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China;Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology,Nanning 530004,China)
出处
《广西大学学报（自然科学版）》
CAS
北大核心
2024年第1期147-160,共14页
Journal of Guangxi University（Natural Science Edition）
基金
广西自然科学基金项目(2022GXNSFDA035062)。
关键词
地聚物
碳酸酐酶
无机膜
二氧化碳捕获
二氧化碳分离
geopolymer
carbonic anhydrase
inorganic membrane
CO_(2)capture
CO_(2)separation
