摘要
目的建立牡丹果荚的质量标准。方法用高效液相色谱法(high performance liquid chromatography,HPLC)测定牡丹果荚中没食子酸、鞣花酸的含量;用薄层色谱法(thin layer chromatography,TLC)鉴别牡丹果荚中没食子酸;按照2020年版《中华人民共和国药典》中的方法测定水分、总灰分、酸不溶性灰分、水溶性浸出物的含量;用生物显微镜观察法对果荚粉末进行鉴别。结果建立了专属性强、重复性好的药材中没食子酸的TLC鉴别方法;建立了药材中没食子酸及鞣花酸含量测定方法:没食子酸在1.30~166.70μg·mL^(−1)范围内线性关系良好(r=0.9995),平均加样回收率为96.66%,RSD值为1.95%;鞣花酸在1.00~128.26μg·mL^(−1)范围内线性关系良好(r=0.9993),平均加样回收率为98.22%,RSD值为1.15%;12批药材中没食子酸、鞣花酸、水分、总灰分、酸不溶性灰分、水溶性浸出物的质量分数分别为7.73~10.00 mg·g^(−1)、11.31~12.50 mg·g^(−1)、9.98%~11.07%、10.35%~12.35%、0.32%~0.41%、32.27%~33.55%;果荚粉末观测到簇晶、导管和非腺毛。结论所建立的牡丹果荚质量标准可用于该药材的质量控制。
Objective To establish the quality standard of the pods of Paeonia ostii T.Hong&J.X.Zhang.Methods High performance liquid chromatography(HPLC)was used to determine the contents of gallic acid and ellagic acid in the pods of Paeonia ostii T.Hong&J.X.Zhang.Thin layer chromatography(TLC)was used to identify gallic acid.The contents of water,total ash,acid insoluble ash and water-soluble extract were determined according to Chinese Pharmacopoeia(2020 Edition).The powders of the pods were identified by biological microscope.Results A TLC identification method of gallic acid with strong specificity and good repeatability was established.Methods for the determination of gallic acid and ellagic acid was also established.Gallic acid has a good relationship in the linear range of 1.30—166.70μg·mL^(−1)with regression coefficient r=0.9995,and its average recovery was 96.66%with RSD 1.95%.The ellagic acid has a good relationship in the range of 1.00—128.26μg·mL^(−1)with regression coefficient r=0.9993,and its average recovery was 98.22%with RSD 1.15%.The mass fraction of gallic acid,ellagic acid,water,total ash,acid insoluble ash and alcohol soluble extract in 14 batches of the pods were 7.73—10.00 mg·g^(−1),11.31—12.50 mg·g^(−1),9.98%—11.07%,10.35%—12.35%,0.32%—0.41%,and 32.27%—33.55%,respectively.Tufts,catheters and non-glandular hairs can be observed in the powder.Conclusion The established HPLC method,TLC method and microscopic method can be used for the quality control of the pods of Paeonia ostii T.Hong&J.X.Zhang.
作者
肖会敏
杨旭
赵利娟
李畅
林奋
王四旺
高锦明
XIAO Huimin;YANG Xu;ZHAO Lijuan;LI Chang;LIN Fen;WANG Siwang;GAO Jinming(College of Chemistry and Pharmacy,Northwest Agriculture and Forestry University,Yangling 712100,China;Research and Development Department of Shaanxi Fengdan Zhengyuan Biotechnology Limited Company,Xi’an 710077,China;Shaanxi Chinese Medicine Institute,Shaanxi Pharmaceutical Information Center,Xianyang 712000,China;Department of Life Science and Medicine,Northwest University,Xi’an,710069,China)
出处
《西北药学杂志》
CAS
2024年第2期10-15,共6页
Northwest Pharmaceutical Journal
基金
陕西省生物医药重点实验室后补助项目(编号:2018SZS41)
陕西省重点研发计划项目(编号:2018SF-315)。
关键词
牡丹
果荚
薄层色谱法
高效液相色谱法
质量标准
没食子酸
鞣花酸
Paeonia ostii T.Hong&J.X.Zhang
pod
thin layer chromatography
high performance liquid chromatography
quality standard
gallic acid
ellagic acid
