摘要
制备牡丹籽油脂质体,以增加牡丹籽油生物利用度和稳定性。采用乙醇注入-超声波法制备牡丹籽油脂质体,并用正交试验设计优化处方和工艺参数。通过荧光倒置显微镜和扫描电镜观察脂质体形态;采用Malvern激光粒度仪测量粒径、多分散系数(PDI)及Zeta电位值;紫外可见分光光度计测量其包封率;同时考察其在4℃和25℃下的稳定性和体外释药特性。结果表明,牡丹籽油脂质体的乙醇注入-超声波法最优工艺为:卵磷脂与胆固醇的质量比为5∶1,牡丹籽油与卵磷脂的质量比为1∶5,缓冲液体积为15 mL,温度为55℃,在此条件下牡丹籽油脂质体包封率达到87.16%、粒径为143.6 nm、PDI为0.217,Zeta电位为-48.3 mV;4℃储存环境中牡丹籽油脂质体的稳定性较好;体外释药结果表明该制剂12 h内累积释放率达80.27%,缓释效果良好。
Preparation of peony seed oil liposomes to increase the bioavailability and stability of peony seed oil.Ethanol injection ultrasonic method was used to prepare peony seed oil liposomes,and orthogonal experimental design was used to optimize the formulation and processing parameters.The morphology of liposome was observed by fluorescence inverted microscope and scanning electron microscope.Particle size,polydispersity index(PDI)and Zeta potential were measured by Malvern laser particle size analyzer.The encapsulation rate was measured by ultraviolet visible spectrophotometer.At the same time,its stability and in vitro release characteristics at 4℃and 25℃were investigated.The results showed that the optimal process of ethanol injection-ultrasonic method was as follows:mass ratio of lecithin to cholesterol 5∶1,mass ratio of peony seed oil to lecithin 1∶5,buffer liquid volume 15 mL,and temperature 55℃.Under the above conditions,the liposome encapsulation rate of peony seed reached 87.16%,particle size was 143.6 nm,PDI was 0.217,and Zeta potential was-48.3 mV.The stability of peony seed oil liposomes at 4℃storage was better.The results of in vitro release showed that the cumulative release rate of the formulation reached 80.27%within 12 h and the sustained-release effect was good.
作者
陈程
闫梦茹
薛守宇
葛鹏辉
吴建洋
杨洁
龚立文
CHEN Cheng;YAN Mengru;XUE Shouyu;GE Penghui;WU Jianyang;YANG Jie;GONG Liwen(Xi’an Medical College,Xi’an 710021)
出处
《中国食品添加剂》
CAS
2024年第3期19-24,共6页
China Food Additives
基金
陕西省科技厅科技计划项目青年项目(2023-JC-QN-0822)
2022年度陕西高校青年创新团队科研计划项目(22JP075)
陕西省科技厅科技计划项目青年项目(2022-JM-568)
陕西高校青年创新团队建设项目(陕教[2019]90号)
西安医学院科技创新团队配套(2021TDPT04)。
关键词
牡丹籽油
脂质体
表征
稳定性
体外释药
peony seed oil
liposome
characterization
stability
in vitro release
