摘要
探究6-甲基-5-庚烯-2-酮(6-methyl-5-hepten-2-one,MHO)处理对砀山酥梨虎皮病关系及对活性氧代谢影响。测定MHO处理砀山酥梨果皮在冷藏过程中α-法尼烯、共轭三烯、MHO、丙二醛、过氧化氢(H_(2)O_(2))、超氧阴离子自由基、总酚含量及过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)和多酚氧化酶(polyphenol oxidase,PPO)的活性,并观察和统计虎皮病的发病情况。结果表明,外源MHO可以诱发相似虎皮病的症状,并且显著增加果皮中MHO、H_(2)O_(2)和超氧阴离子自由基的含量,降低抗氧化酶CAT、POD和SOD的活性,增加了α-法尼烯、共轭三烯的含量。果皮MHO含量与H_(2)O_(2)和超氧阴离子自由基含量呈极显著相关,但果皮的MHO含量比H_(2)O_(2)与虎皮病发病率的关系更密切,这些结果表明MHO可能通过增加活性氧的积累而引发梨虎皮病。
In this study,the relationship between 6-methyl-5-hepten-2-one(MHO)treatment and superficial scald in‘Dangshansuli’pear fruit and its impact on reactive oxygen species(ROS)metabolism were explored.The levels ofα-farnesene,conjugated trienes,MHO,malondialdehyde(MDA),hydrogen peroxide(H_(2)O_(2)),superoxide anion radical and total phenols,and the activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD)and polyphenol oxidase(PPO)in the pericarp of‘Dangshansuli’pear fruit treated by MHO during cold storage were measured.In addition,the development of superficial scald was observed and recorded.The results indicated that exogenous MHO could induce symptoms similar to superficial scald in‘Dangshansuli’pears,significantly increase the contents of MHO,H_(2)O_(2) and superoxide anion radical,and decrease the activities of the antioxidant enzymes CAT,POD and SOD,and increase the contents ofα-farnesene and conjugated trienes.The content of MHO in the pericarp was significantly related to the contents of H_(2)O_(2) and superoxide anion radical,but was more closely correlated with the incidence rate of superficial scald than H_(2)O_(2) levels.These results reveal that MHO can induce superficial scald by increasing the accumulation of ROS.
作者
崔子璇
高旭
常姗
杨晓露
惠伟
CUI Zixuan;GAO Xu;CHANG Shan;YANG Xiaolu;HUI Wei(College of Life Sciences,Shaanxi Normal University,Xi’an 710119,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第5期250-256,共7页
Food Science
基金
陕西省林业科技创新计划项目(SXLK2021-0228)
陕西省农业科技创新驱动集成推广项目(NYKJ-2020-XA-03)。
关键词
虎皮病
活性氧
6-甲基-5庚烯-二-酮
梨
superficial scald
reactive oxygen species
6-methyl-5-hepten-2-one
pear
