摘要
为优化甘薯种质抗茎腐病鉴定技术,通过分析不同的接种方法和菌液浓度对甘薯种质抗性的影响,建立甘薯抗茎腐病鉴定标准,为甘薯抗病育种奠定基础。利用组培瓶蛭石薯苗擦伤接菌法和最佳接种菌液浓度(1.1×10^(7)CFU·mL^(-1)),对最具代表性的6个甘薯品种进行接种鉴定,并与田间抗性品种比较进行验证,室内接种抗性鉴定结果与田间抗性表现基本一致,表明新抗性鉴定技术的鉴定结果具有准确性、可靠性。进一步对65个甘薯材料进行抗病鉴定,发现绝大部分材料表现为感病或中感,7份种质(YD7002、DY7082-1、浙紫薯1号、TF1118、DY7032、南薯88和浙紫薯4号)对甘薯茎腐病具有稳定抗性。文章建立了甘薯抗茎腐病的新鉴定技术,鉴定和筛选出对甘薯茎腐病表现稳定抗性的种质,为甘薯抗茎腐病育种提供重要的种质资源,对于甘薯病害防控具有重要意义。
In order to optimize the identification technique for stem rot resistance in sweet potato germplasm,this study analyzed the effects of different inoculation methods and bacterial liquid concentrations on the resistance of sweet potato germplasm.The aim was to establish a standardized identification criterion for stem rot resistance and lay a foundation for disease-resistant breeding in sweet potato.Using the inoculation method of scratching the surface of tissue-cultured sweet potato plantlets and the optimal bacterial liquid concentration(1.1×10^(7)CFU·mL^(-1)),six representative sweet potato varieties were inoculated and identified.The results of indoor resistance identification were validated against field resistance performance and showed a high degree of consistency,indicating the accuracy and reliability of the newly developed identification technique.Furthermore,65 sweet potato materials were identified for resistance to stem rot.Most of the materials were susceptible or moderately susceptible.However,seven germplasm resources(YD7002,DY7082-1,Zhezi No.1,TF1118,DY7032,Nanshu 88 and Zhezi No.4)exhibited stable resistance against stem rot in sweet potato.This study established a new identification technique for stem rot resistance in sweet potato and identified germplasm resources with stable resistance,providing important genetic materials for disease-resistant breeding.These findings are of significant importance for the prevention and control of sweet potato diseases.
作者
柴荣耀
游雨欣
邱海萍
郭峻宁
张震
李斌
沈升法
王艳丽
CHAI Rongyao;YOU Yuxin;QIU Haiping;GUO Junning;ZHANG Zhen;LI Bin;SHEN Shengfa;WANG Yanli(Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences/State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products/Zhejiang National Crop Variety Resistance Identification Experiment Station,Hangzhou 310021,China;State Key Laboratory of Rice Biology and Breeding,Key Laboratory of Molecular lar Biology of Crop Pathogens and Insects,Ministry of Agriculture and Rural Affairs,Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institure of Biotechnology,Zhejiang University,Hangzhou 310058,China;Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
出处
《浙江农业学报》
CSCD
北大核心
2024年第3期569-578,共10页
Acta Agriculturae Zhejiangensis
基金
浙江省重点研发计划(2017C02002)
浙江省农业科学院省部共建农产品质量安全危害因子与风险防控国家重点实验室自主设计项目(2010DS700124-ZZ2014)。
关键词
甘薯
茎腐病
种质资源
抗性鉴定
sweet potato
stem rot
germplasm resource
resistance identification
