摘要
目的聚精助育汤为临床治疗不育症的自拟复方,疗效确切,其药效机制尚未阐明。方法利用TCMSP数据库收集聚精助育汤的化合物及其靶点,利用Gencards数据库收集不育症相关疾病靶点,利用STRING11.0数据库分析聚精助育汤-不育症共同靶点的蛋白质相互作用(PPI),应用Cystoscape 3.8.0软件进行核心基因的筛选,GO的生物过程(biological process,BP)、分子功能(molecular function,MF)、细胞组分(cell component,CC)富集分析,使用String数据库,使用R 4.0.3软件,绘制气泡图、柱状图。将成分-疾病-通路-靶点等导入系统进行通路网络图的绘制。结果经过检索、筛选,共获得聚精助育汤化合物248个,化合物潜在靶点2238个,不育症相关疾病基因1369个,聚精助育汤-不育症共同靶点185个。网络药理学分析结果表明,槲皮素、木犀草素、山奈酚、γ-氨基丁酸等是聚精助育汤的主要活性成分,ESR1、IL2、FOS、NFE2L2等是聚精助育汤治疗不育症的主要靶点,共同靶点富集于PI3K-AKt等多个信号通路,调节细胞、凋亡、基因转录等生物学过程。结论初步确定了聚精助育汤治疗不育症的机制及作用靶点,为后续研究提供参考。
Objective Jujing Zhuyu decoction is a self-made compound for the clinical treatment of male infertility.Its curative effect is definite,and its mechanism of action has not been clarified.Methods Using the TCMSP database to collect compounds and targets of Jujing Zhuyu decoction,using the gencards database to collect targets of infertility related diseases,using the STRING 11.0 database to analyze protein interactions(PPI)between Jujing Zhuyu decoction and the common target of infertility,using Cytoscape 3.8.0 software to screen core genes,biological processes(BP)and molecular functions(MF)of GO cell component(CC)enrichment analysis was performed using the string database and R 4.0.3 software to draw bubble and bar charts.Import components,diseases,pathways,targets,etc.into the system to draw a pathway network diagram.Results After searching and screening,248 compounds of Jujing Zhuyu decoction were obtained,including 2238 potential targets,1369 genes related to male infertility,and 185 common targets of Jujing Zhuyu decoction and male infertility.The results of network pharmacological analysis showed that quercetin,luteolin,kaempferolγ-Aminobutyric acid is the main active component of Jujing Zhuyu decoction.ESR1,IL2,Fos and nfe2l2 are the main targets of Jujing Zhuyu decoction in the treatment of male infertility.The common targets are enriched in multiple signal pathways such as PI3K-Akt,and regulate biological processes such as cell,apoptosis and gene transcription.Conclusion The mechanism and target of action of Jujing Zhuyu decoction in treating infertility have been preliminarily determined,providing reference for subsequent research.
作者
傅伟
徐磊
林美欣
李晨曦
游旭军
秦国政
FU Wei;XU Lei;LIN Meixin;LI Chenxi;YOU Xujun;QIN Guozheng(Seventh Clinical Medical College,Guangzhou University of Chinese Medicine,Shenzhen 518133,China;The First Clinical Medical College of Yunnan University of Chinese Medicine,Kunming 650500,China;Henan Traditional Chinese Medicine Hospital,The Second Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China;Yunnan Traditional Chinese Medicine Hospital,The First Affiliated Hospital of Yunnan University of Chinese Medicine,Kunming 650021,China)
出处
《云南中医药大学学报》
2024年第1期98-106,共9页
Journal of Yunnan University of Chinese Medicine
基金
全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号)
国家自然科学基金项目(81260540,81760872,82104853)
深圳市宝安中医院资助项目(BAZYY20200610)。
关键词
聚精助育汤
不育症
网络药理学
Jujing Zhuyu decoction
infertility
network pharmacology
