摘要
目的 探讨外周血循环肿瘤细胞(CTC)、程序性死亡因子配体-1(PD-L1)、及钙周期素结合蛋白(S100A6BP)水平对胃癌患者诊断及预后价值研究。方法 选取2020年5月至2022年5月南阳市第一人民医院收治的182例胃癌患者为观察组,根据预后情况分为预后良好组(124例)及预后不良组(58例),选取同期90名健康志愿者作为对照组。比较所有受试者外周血CTC、PD-L1、S100A6BP水平,评估CTC、PD-L1、S100A6BP单独及联合检测在胃癌患者诊断及预后中的应用价值。结果 观察组外周血CTC、PD-L1及S100A6BP水平均高于对照组,差异有统计学意义(P<0.05);CTC、PD-L1及S100A6BP三者联合检测对胃癌患者诊断灵敏度、特异度均较单一检测高(P<0.05);预后良好者肿瘤直径、CTC、PD-L1、S100A6BP、TNM(Ⅲ~Ⅳ)、中高分化、淋巴结转移、浸润深度(T3-T4)均较预后不良组低,差异有统计学意义(P<0.05),TNM(Ⅰ~Ⅱ)、低度分化、浸润深度(T1-T2)水平较预后不良组高,差异有统计学意义(P<0.05);多因素Logistic回归分析显示,肿瘤直径、TNM分期、分化程度、浸润深度、淋巴结转移、CTC、PD-L1、S100A6BP是影响胃癌预后不良的独立危险因素(P<0.05);ROC曲线分析显示,CTC、PD-L1、S100A6BP单一及联合检测预测胃癌患者预后AUC分别为0.808、0.845、0.874、0.955(P<0.05)。结论 CTC、PD-L1、S100A6BP在胃癌患者外周血中呈高表达,与患者病理特征及预后有密切关系,三者联合检测对胃癌患者诊断及预后具有较高的预测价值。
Objective To investigate the diagnostic and prognostic value of peripheral blood circu-lating tumor cells(CTC),programmed death factor ligand-1(PD-L1),and calcyclin binding protein(S100A6BP)levels in patients with gastric cancer.Methods A total of 182 patients with gastric cancer were admitted to Nanyang First Peoples Hospital from May 2020 to May 2022 and were selected as the observation group.Based on their prognosis,they were divided into two groups:the good prognosis group(124 cases)and the poor prognosis group(58 cases).Additionally,90 healthy volunteers were chosen as the control group during the same period.The levels of CTC,PD-L1 and S100A6BP in the peripheral blood of all subjects were compared,and the diagnosis and prognosis value of CTC,PD-L1 and S100A6BP alone and combination were evaluated for patients with gastric cancer patients.Results The levels of CTC,PD-L1 and S100A6BP in the peripheral blood of the observation group were higher than those of the control group,with statistical signifi-cance(P<0.05).The sensitivity and specificity of CTC,PD-L1 and S100A6BP in diagnosing gastric cancer was higher than that of single detection(P<0.05).In the good prognosis group,the tumor diameter,CTC,PD-L1,S100A6BP,TNM(Ⅲ~Ⅳ),middle and high differentiation,lymph node metastasis,and depth of in-vasion(T3-T4)were all significantly lower than those in the poor prognosis group(P<0.05).Conversely,the levels of TNM(Ⅰ~Ⅱ),low differentiation and depth of invasion(T1-T2)were significantly higher in the good prognosis group,compared to the poor prognosis group(P<0.05).Multivariate logistic regression analy-sis showed that tumor diameter,TNM stage,degree of differentiation,depth of invasion,lymph node metasta-sis,CTC,PD-L1 and S100A6BP were independent risk factors for poor prognosis of gastric cancer(P<0.05).The ROC curve analysis demonstrated that the prognostic AUC of CTC,PD-L1 and S100A6BP single and combined detection was 0.808,0.845,0.874 and 0.955,respectively(P<0.05).Conclusion CTC,PD-L1 and S100A6BP are high
作者
徐毅
唐岩
李冬扬
XU Yi;TANG Yan;LI Dongyang(The Second Ward of Gastrointestinal Surgery,the First Peoples Hospital of Nanyang,Nanyang,Henan,China,473010)
出处
《分子诊断与治疗杂志》
2024年第2期273-277,共5页
Journal of Molecular Diagnostics and Therapy
基金
河南省医学科技攻关计划项目(LHGJ20200902)。
