摘要
目的 分析贵州省某院128例新型冠状病毒感染患者血清HSP 70表达水平及HSP 70-1基因多态性变化,并探讨其临床价值。方法 选取2020年1月至2020年2月贵州省某医院收治的128例新型冠状病毒感染患者(病例组),同期128名健康体检者(对照组)。采用酶联免疫吸附试验(ELISA)比较两组血清中HSP 70水平变化;采用单核苷酸多态性(SNP)基因分型方法检测HSP 70-1(+190位点)基因序列,分析基因多态性。结果 病例组患者血清中HSP 70蛋白高于对照组,差异有统计学意义(Z=6.924,P<0.05),并且随着病例组患者病程的加重,血清HSP 70水平也逐渐升高,各临床分型的HSP70水平均高于对照组,差异均有统计学意义(P<0.05);HSP 70-1(+190位点)的基因型GG、GC、CC在患者病例组均存在,与对照组比较,差异有统计学意义(χ^(2)=6.975,P<0.05),此外,轻型患者HSP 70-1 GC基因型的分布频率(59.26%)及普通型患者HSP 70-1 GC基因型的分布频率(52.69%)均高于对照组(39.84%)(P<0.05),而轻型患者HSP 70-1 GG基因型的分布频率(33.33%)及普通型患者HSP 70-1 GG基因型的分布频率(38.71%)均低于对照组(53.90%),差异有统计学意义(P<0.05)。结论 HSP 70表达水平及HSP 70-1基因分型与新型冠状病毒感染的发生、发展相关,可辅助新型冠状病毒感染患者临床分型的判断。
Objective To investigate the changes in serum HSP 70 expression and its relationship with HSP 70-1 gene polymorphism in 128 patients with novel coronavirus infection at a hospital in Guizhou Province.Methods A total of 128 patients with novel coronavirus infection(case group)and 128 healthy subjects(control group)who were admitted to a hospital in Guizhou Province from January 2020 to February 2020 were selected for the study.The changes in serum HSP 70 levels between the two groups were compared using enzyme-linked immunosorbent assay(ELISA).The single nucleotide polymorphism(SNP)genotyping method was used to detect the HSP70-1 gene sequence at the+190 locus and analyze gene polymorphism.Results The serum HSP 70 protein levels in the case group were higher than those in the control group,with a statistically significant difference(Z=6.924,P<0.05).Additionally,the serum HSP 70 levels in the case group increased gradually as the disease progressed.The HSP 70 levels in each clinical classification were high-er than those in the control group,and the differences were statistically significant(P<0.05).In the patient case group,HSP 70-1(+190 locus)genotypes GG,GC and CC were identified,with a statistically signifi-cant difference compared to the control group(χ^(2)=6.975,P<0.05).In addition,the distribution frequency of the HSP 70-1 GC genotype in mild patients(59.26%)and common patients(52.69%)was higher than that in the control group(39.84%)(P<0.05).Conversely,the distribution frequency of the HSP 70-1 GG genotype in mild patients(33.33%)and common patients(38.71%)was lower than that in the control group(53.90%),with a statistically significant difference(P<0.05).Conclusion The expression levels of HSP 70 and the HSP 70-1 genotype are related to the occurrence and development of novel coronavirus infection.This can help in determining the clinical classification of patients with novel coronavirus infection.
作者
牟丽丽
黄山
夏婧
MU Lii;HUANG Shan;XIA Jing(Guizhou Nursing Vocational College,Guiyang,Guizhou,China,550025)
出处
《分子诊断与治疗杂志》
2024年第3期467-471,共5页
Journal of Molecular Diagnostics and Therapy
基金
贵州省卫生健康委科学技术基金项目(gzwjkj2020-1-062)。
