摘要
为探究紫锥菊提取物-菊苣酸在低氧环境下影响SD大鼠肝脏脂肪代谢的分子机制。选取60只健康雄性SD大鼠(6~8周龄,(192±7.35)g)(P>0.05),适应性喂养2周后,随机分为对照组、不同浓度CA组(10、20、40 mg/kg),每组15只,连续灌胃49 d。4组大鼠均自由采食和饮水,灌胃第50天对SD大鼠进行麻醉处理后,采集肝脏组织,并通过油红O染色法计算各组大鼠肝脏组织油滴面积占总面积的百分比以及测定PPAR信号通路关键因子PLIN2、PLIN5、PPARα、PPARγ、RXRα的表达情况。油红O染色结果显示:与对照组相比,添加紫锥菊提取物-菊苣酸组极显著降低油红O染色面积(P<0.01);PPAR信号通路关键因子表达结果显示:与对照组相比,添加紫锥菊提取物-菊苣酸组显著降低PLIN2、PLIN5、PPARα、PPARγ、RXRα的mRNA及蛋白表达量(P<0.05),其中以添加10 mg/kg紫锥菊提取物-菊苣酸组对PLIN2、PLIN5、PPARα、PPARγ、RXRα的mRNA及蛋白表达量效果最显著(P<0.05)。结果表明,紫锥菊提取物-菊苣酸降低脂肪合成相关因子的表达,调控脂肪的合成与储存,预防脂肪的过多累积导致机体脂质代谢紊乱,紫锥菊提取物-菊苣酸改善了低氧诱导的脂质代谢功能障碍,且10 mg/kg组作用效果最好。本研究为紫锥菊提取物-菊苣酸作为高海拔地区预防高原动物脂质代谢紊乱的饲料添加剂的开发利用提供了理论基础。
The aim of this study is to explore the molecular mechanism of Echinacea purpurea extract-chicric acid effect on hepatic fat metabolism in SD rats.Sixty 6-8 weeks old healthy male SD rats(192±7.35)g were randomly divided into the control and CA groups(10,20,40 mg/kg)after 2 weeks of adaptive feeding.The liver tissues of the rats in each group were collected and the percentage of the total area of oil droplets and the expression of the key factors of the PPAR signaling pathway including PLIN2,PLIN5,PPARα,PPARγand RXRα,were measured by Oil-Red O staining.The results of Oil-Red O staining showed that the area of staining was significantly reduced in the group with the addition of Echinacea extract-chicory acid compared with the control group(P<0.01);the results of expression of key factors of PPAR signaling pathway showed that the expression of PLIN2,PLIN5,PPARα,PPARγand RXRαwas significantly reduced in the group with the addition of Echinacea extract-chicory acid compared with the control group(P<0.05).The effect of adding 20 mg/kg of Echinacea extract-chicory acid on the mRNA and protein expression of PLIN2,PLIN5,PPARα,PPARγand RXRαwas the most significant(P<0.05).In conclusion,Echinacea extract-chicory acid reduced the expression of lipid synthesis-related factors,regulated lipid synthesis and storage,and prevented the excessive accumulation of fat leading to the disorder of lipid metabolism in the body,and Echinacea extract-chicory acid improved the dysfunction of lipid metabolism induced by hypoxia,and the 20 mg/kg group had the best effect.This study may provide a theoretical basis for the development and utilization of Echinacea extract-chicric acid as a feed additive to prevent lipid metabolism disorders in highland animals at high altitude.
作者
行倩文
吴华
刘嘉华
刘波
张龙飞
陈鑫磊
马欣茹
XING Qianwen;WU Hua;LIU Jiahua;LIU Bo;ZHANG Longfei;CHEN Xinlei;MA Xinru(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2024年第1期135-140,共6页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(32160812)。
