摘要
目的 探讨葛根素(Puerarin)对葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导溃疡性结肠炎(ulcerative colitis,UC)小鼠的改善作用及机制。方法 2.5%DSS溶液被灌以小鼠进而建造UC模型,将40只小鼠随机分组,分为正常组、模型组、葛根素低剂量(20 mg/kg·d)、中剂量(30 mg/kg·d)和高剂量(40 mg/kg·d),连续给药8 d,对小鼠的体重变化、疾病指数(DAI)的变化、结肠长度等进行评价,苏木精-伊红染色法处理评价结肠组织损伤程度、酶联免疫吸附测定试剂盒定量检测小鼠结肠组织中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、髓过氧化物酶(MPO)、一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)的水平。结果 葛根素可减缓小鼠体重下降的趋势,降低小鼠疾病活动指数,延缓结肠长度缩短及降低结肠组织损伤的程度。初步作用机制研究表明,葛根素各剂量组能不同程度地降低结肠组织中促炎因子(IL-1β、TNF-ɑ和IL-6)水平,降低MPO、NO、iNOS水平。结论 葛根素对DSS诱导的UC小鼠具有改善作用,其作用机制可能与抗炎、抗氧化有关。
Objective To investigate the effect of puerarin on ulcerative colitis(UC)induced by dextran sodium sulfate(DSS)in mice and its mechanisun.Methods 2.5%DSS solution was administered to mice to construct UC model.40 mice were randomly divided into normal group,model group,low dose group(20mg/kg·d),medium dose group(30mg/kg·d)and high dose group(40mg/kg·d).After administration for 8 days,the changes of body weight,disease index(DAI)and colon length were evaluated.The degree of colon tissue injury was evaluated by hematoxylin-eosin staining,and the levels of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),myeloperoxidase(MPO),nitric oxide(NO)and inducible nitric oxide synthase(iNOS)in colon tissues of mice were quantitatively detected by enzyme-linked immunosorbent assay kit.The level of synthase(iNOS).Results Puerarin could slow down the trend of weight loss,decrease the disease activity index,delay the shortening of colon length and reduce the degree of colon tissue injury.Preliminary study on the mechanism showed that different puerarin dose groups could reduce the levels of IL-6,IL-1β,IL-α,MPO,NO and iNOS.Conclusion Puerarin has protective effect on DSS induced UC mice,and its mechanism may be related to anti-inflammation and anti-oxidation.
作者
邵骏菁
孙芳
许昊
杨梦涵
由智栋
李会
周晓娅
史才兴
SHAO Junjing;SUN Fang;XU Hao;YANG Menghan;YOU Zhidong;LI Hui;ZHOU Xiaoya;SHI Caixing(Jining Medical University,Jining 272067,Shandong)
出处
《菏泽医学专科学校学报》
2024年第1期1-5,10,共6页
Journal of Heze Medical College
基金
山东省医药卫生科技发展计划项目(202202040316)
山东中医药科技项目(Q-2023072)
济宁医学院2020年校级教育科学研究项目(Y2020043)。
关键词
葛根素
溃疡性结肠炎
葡聚糖硫酸钠
炎症
Puerarin
Ulcerative colitis
Dextran sodium sulfate
Inflammation
