摘要
【目的】分析永泰县茶树种质资源的遗传多样性及群体结构,探明不同茶树资源群体的亲缘关系,为当地茶树种质资源的收集和优异种质利用提供依据。【方法】利用EST-SNP分子标记技术对77份永泰及其周边地区茶树种质资源进行亲缘关系和遗传多样性分析。【结果】筛选出48个适用于鉴定永泰茶树种质的SNP位点。这些SNP位点的多态性信息指数平均值为0.407,观测杂合度平均值为0.303,期望杂合度平均值为0.271,固定指数平均值为-0.092,次等位基因频率平均值为0.268。通过主坐标分析、聚类分析和Structure群体结构分析,发现永泰茶树种质的同一群体内部的交流多于不同群体之间的交流,且梧桐的茶树种质与洑口、丹云等地的种质差异较大,两者间的遗传距离较远,但又与尤溪和大田的茶树亲缘关系较近。此外,应用筛选出的48个高质量SNP位点构建了54份永泰茶树种质的DNA指纹图谱,可用于相关茶树种质的精确鉴定。【结论】永泰县茶树种质资源多样性丰富;永泰县茶树种质资源按照地理位置可划分为梧桐镇产区和其他地区两个类群;梧桐茶树资源与大田和尤溪的茶树资源亲缘关系较为密切,而洑口、丹云等地的茶树资源与永泰县北部产茶地的茶树资源亲缘关系更近。
【Objective】Genetic diversity and population structure of tea germplasms in Yongtai County were analyzed for resource utilization.【Method】The EST-SNP molecular marker technology was applied to analyze the genetic relationships and diversity of 77 tea germplasms collected from Yongtai and surrounding areas.【Result】Forty-eight SNP loci for identifying the germplasms were selected.The average polymorphism information index of the loci was 0.407 with an average observed heterozygosity of 0.303 and an expected one of 0.271,as well as the average fixed index of−0.092 and the frequency of secondary alleles of 0.268.The principal coordinate,cluster,and structure population structure analyses revealed that the interactions within a same population of the tea plants were stronger than among different populations at Yongtai,and that the tea germplasms found in Wutong significantly differed from those in Fukou,Danyun,and other places with significant genetic distance but close to those from Youxi and Datian.Using the 48 selected high-quality SNP loci,a library of the DNA fingerprints on 54 germplasms from Yongtai was established to provide a means for accurate germplasm identification.【Conclusion】Yongtai County was rich in the resource of varieties of tea plants,which could be classified geographically into those from Wutong and those from Fukou,Danyun and other localities in the county.The cultivars from Wutong were more closely related to those found in Datian and Youxi,whereas those from Fukou,Danyun,etc.more to those existed in the north part of the county.
作者
王攀
于文涛
吕水源
朱艳宇
兰振良
李青颖
李国来
贵文静
叶乃兴
WANG Pan;YU Wentao;LV Shuiyuan;ZHU Yanyu;LAN Zhenliang;LI Qingying;LI Guolai;GUI Wenjing;YE Naixing(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Technology Centre of Fuzhou Customs/Fujian Key Laboratory for Technology Research of Inspection and Quarantine,Fuzhou,Fujian 350001,China;Fujian Lufeng Tea Industry Co.,Ltd.,Yongtai,Fujian 350700,China;Zishan Tianming Tea Professional Cooperative in Yongtai,Yongtai,Fujian 350704,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2024年第1期66-74,共9页
Fujian Journal of Agricultural Sciences
基金
福建省农业科技引导性项目(2021N0024)
海关总署科研项目(2020HK187)
福建农林大学横向课题(KH220008A)
福建张天福茶叶发展基金会科技创新基金(FJZTF01)。
