摘要
目的探讨吩噻嗪类单体化合物(PCA)抑制衣原体生长的效果。方法建立体外衣原体培养基,以HeLa S3细胞为载体,扩增衣原体CtL2,CtD,MoPn种属,同时探讨PCA及其衍生物对上述衣原体种属的生长抑制作用及安全性。结果PCA对CtL2,CtD,MoPn均有抑制作用,其中CtL2的半数抑制浓度(IC_(50))与IC_(95)分别为4.30μmol和12.16μmol;CtD相关IC_(50)与IC_(95)分别为3.87μmol和7.69μmol;MoPn相关IC_(50)与IC_(95)分别为1.64μmol和95μmol,且与浓度存在依赖性;进一步分析吩噻嗪类衍生物S1,S2,S3抗支原体活性时发现,S3抗衣原体活性为PCA的40倍;最后,荧光显示了PCA处理组细胞的形态和核的形状均未改变,且细胞数量也未减少。结论PCA及其衍生物具有良好的抗衣原体活性,且与浓度呈正相关,同时并未破坏细胞的整体结构,具有潜在的临床治疗衣原体感染的意义。
Objective To investigate the inhibitory effect of phenothiazine monomers(PCA)on the growth of Chlamydia.Methods To establish an in vitro culture medium for Chlamydia,HeLa S3 cells were used as carriers to amplify Chlamydia CtL2,CtD,and MoPn species.Meanwhile,the growth inhibitory effects and safety of PCA and its derivatives on the aforementioned Chlamydia species were investigated.Results PCA had inhibitory effects on CtL2,CtD,and MoPn,with the half inhibitory concentration(IC_(50))and IC_(95)of CtL2 being 4.30μmol and 12.16μmol,respectively.The IC_(50)and IC_(95)related to CtD were 3.87μmol and 7.69μmol,respectively.The IC_(50)and IC_(95)related to MoPn were 1.64μmol and 95μmol,respectively,and there was a concentration dependence.Further analysis of the anti-mycoplasma activity of phenothiazine derivatives S1,S2,and S3 revealed that the anti Chlamydia activity of S3 was 40 times higher than that of PCA.Finally,fluorescence showed that the morphology and nuclear shape of the cells in the PCA treated group remained unchanged,and the number of cells did not decrease.Conclusion PCA and its derivatives have good anti Chlamydia activity and are positively correlated with concentration,while not damaging the overall structure of cells,which has potential clinical significance for the treatment of Chlamydia infection.
作者
陈丹丹
金祥
许阿兰
倪凤燕
葛建彬
CHEN Dandan;JIN Xiang;XU Alan;NI Fengyan;GE Jianbin(Department of Pharmacy,The Second People's Hospital of Nantong City,Nantong,Jiangsu,China 226002)
出处
《中国药业》
CAS
2023年第S02期1-4,共4页
China Pharmaceuticals
基金
江苏省药学会天晴医院药学课题[Q2019157]。
关键词
衣原体
吩噻嗪类单体
衍生物
细胞结构
细胞培养
Chlamydia
phenylthiazide monomers
derivatives
cellular structure
cell culture
