摘要
目的以健康和膝骨性关节炎(Knee osteoarthritis,KOA)模型大鼠为载体,研究针刺对不同机能状态大鼠足三里穴敏化情况的影响。方法将雄性SD大鼠随机分为空白组、模型组、模针组和空针组,每组7只,模型组和模针组采用1 mg·50μL-1单碘乙酸盐关节腔内注射制备KOA大鼠模型。于造模第2天对模针组和空针组大鼠左侧足三里穴行针刺治疗,每天1次,每次20 min,5次为一疗程,进行2个疗程,疗程之间间隔2天。在造模前和针刺结束后观察大鼠一般情况,测量大鼠膝关节直径、足底热痛阈值、进行Lequesne MG评分、观察膝关节软骨形态学判断模型是否成功,测量大鼠足三里穴机械痛阈值观察穴位敏化情况,进行穴区皮肤肥大细胞(Mast cells,MC)形态观察和计数、检测穴区皮肤降钙素相关肽(Calcitonin gene-related peptide,CGRP)蛋白表达情况。结果与造模前相比,模型组和空针组针刺后足三里穴机械痛阈明显降低(P<0.01,P<0.05)。与空白组相比,模型组和空针组足三里穴机械痛阈变化率明显增大(P<0.05);与模型组相比,模针组足三里穴机械痛阈变化率明显降低(P<0.01)。与空白组相比,模型组足三里穴皮肤组织CGRP蛋白荧光强度明显升高(P<0.01),MC脱颗粒率明显增高(P<0.05),空针组足三里穴皮肤组织CGRP蛋白荧光强度无显著差异(P>0.05),MC脱颗粒率明显增高(P<0.01)。与模型组相比,模针组足三里穴皮肤组织CGRP蛋白荧光强度明显减少(P<0.01),MC脱颗粒率无明显差异(P>0.05)。结论不同机能条件下,针刺对足三里状态的影响不同:在生理状态下针刺可激发穴位出现类敏化现象,疾病状态下针刺消减疾病诱发穴位敏化实现治疗作用。
Objective To investigates the effect of acupuncture on sensitization of Zusanli(ST36)in rats with different functional states by using healthy and knee osteoarthritis model rats.Methods Male SD rats were randomly divided into control,model,model-acupuncture and blank-acupuncture group,with 7 rats in each group.KOA rat model was prepared by intra-articular injection of 1 mg·50μL-1 monoiodoacetic aci(MIA)in model group and model-acupuncture group.On the second day of modeling,acupuncture treatment was performed on the left Zusanli of the model acupuncture group and the blank-acupuncture group,once everyday for 20 min,5 times as a course of treatment,2 days between courses.The general condition,knee joint diameter,plantar thermal pain threshold and Lequesne MG score of rats was observed before modeling and after acupuncture.Observing the morphology of knee joint cartilage to judge whether the model is successful,measuring the mechanical pain threshold of Zusanli to investigate the acupoint sensitization,observing and counting the morphology of skin mast cells in the acupoint area,and detecting the expression of skin calcitonin gene-related peptide(CGRP)in the acupoint area.Results The mechanical pain threshold of Zusanli after acupuncture in model group and blank-acupuncture group decreased significantly after modeling(P<0.01,P<0.05),compared with the control group,the change rate of mechanical pain threshold in model group and blank-acupuncture group increased significantly(P<0.05),compared with the model group,the mechanical pain threshold of Zusanli in the model-acupuncture group decreased significantly(P<0.01).Compared with the control group,the fluorescence intensity of CGRP protein in the skin tissue of Zusanli in the model group increased significantly(P<0.01),MC degranulation rate increased significantly(P<0.05),and there was no significant difference in the fluorescence intensity of CGRP protein of Zusanli in the blank-acupuncture group(P>0.05),MC degranulation rate increased obviously(P<0.01),CGRP protei
作者
罗新叶
刘柏彤
曾灵活
郑宇琦
齐文川
蔡定均
Luo Xinye;Liu Baitong;Zeng Linghuo;Zheng Yuqi;Qi Wenchuan;Cai Dingjun(College of Acupuncture and Massage,Chengdu University of Traditional Chinese Medicine,Chengdu 610036,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第12期4039-4046,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会面上项目(8227152930):基于Bmal1-KLF15研究针刺促进缺血心肌修复整体性调节的生物钟机制,负责人:蔡定均
四川省科学技术厅科技计划项目(2019YJ0330):外泌体介导的穴位敏化肥大细胞-神经调控机制研究(省重),负责人:蔡定均
四川省科学技术厅科技计划项目(2021YJ0177):基于SP-Mrgprb2R研究择时针刺治疗神经病理性疼痛的钟控神经-免疫调节机制,负责人:赵征宇。
关键词
穴位敏化
针刺
机能状态
膝骨性关节炎
Acupoint sensitization
Acupuncture
Functional state
Knee osteoarthritis
