摘要
为了运用网络药理学和分子对接技术分析车前草的药理活性及其防治畜禽腹泻的作用机制,本试验利用传统中药系统药理学分析平台(TCMSP)获得车前草的有效活性成分和靶点,利用Cytoscape 3.7.1软件构建车前草-活性成分-靶点网络;通过GeneCards和OMIM数据库查询腹泻疾病靶点,提取药物与疾病交集靶点;通过STRING数据库对交集靶点进行蛋白质相互作用网络(PPI)分析,使用Cytoscape 3.7.1软件进行可视化处理绘制PPI图;利用DAVID数据库对靶点进行富集分析;通过分子对接技术对有效活性成分和关键靶点进行对接验证。结果显示,车前草活性成分有木犀草素、黄芩素、豆甾醇、高车前素、6-羟基木犀草素、谷甾醇和黄芩苷,可作用于α-丝氨酸/苏氨酸蛋白激酶(AKT1)、肿瘤蛋白P53(TP53)、肿瘤坏死因子(TNF)、半胱氨酸天冬氨酸蛋白酶-3(CASP3)、白细胞介素6(IL-6)、表皮生长因子受体(EGFR)、细胞周期蛋白D1(CCND1)和基质金属蛋白酶9(MMP9)等关键靶点;GO功能富集分析显示与生物过程相关的条目154条,与细胞组分相关的条目55条,与分子功能相关的条目105条;KEGG通路条目82条,主要参与调控癌症和磷脂酰肌醇-3激酶-丝氨酸/苏氨酸蛋白激酶(PI3K-AKT)信号通路;车前草的有效活性成分木犀草素和黄芩素与关键靶点AKT1可稳定结合。结果表明,车前草可以通过木犀草素、黄芩素、豆甾醇和高车前素等多种活性成分作用于AKT1、TP53、TNF、CASP3和IL-6等关键靶点上,通过参与多种癌症和PI3K-AKT信号通路的调控防治畜禽腹泻。
In order to analyze the pharmacological activity and the mechanism of Plantago asiatica L.in preventing and treating livestock and poultry diarrhea using network pharmacology and molecular docking technology,this study utilized the Traditional Chinese Medicine Systems Pharmacology(TCMSP)analysis platform to obtain active components and targets of Plantago asiatica L.Plantago asiatica L.-active component-target network was constructed using Cytoscape 3.7.1 software.Targets related to diarrhea diseases were obtained from the GeneCards and OMIM databases,and the intersection targets between drugs and diseases were extracted.The protein-protein interaction network(PPI)analysis of intersection targets was conducted using the STRING database,and the PPI network was visualized and processed using Cytoscape 3.7.1 software.Enrichment analysis of targets was performed using the DAVID database.Molecular docking technology was employed to dock and verify the active components and key targets.The results showed that the active components of Plantago asiatica L.included luteolin,baicalin,stigmasterol,dinatin,6-hydroxyluteolin,sitosterol,and baicalin.These components acted on key targets such as AKT1,TP53,TNF,CASP3,IL-6,EGFR,CCND1,and MMP9.Gene Ontology(GO)enrichment analysis revealed 154 entries related to biological processes,55 entries related to cellular components,and 105 entries related to molecular functions.The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis identified 82 entries,primarily involved in the regulation of cancer and the phosphatidylinositol 3-kinase-serine/threonine-protein kinase(PI3K-AKT)signaling pathway.The effective active components luteolin and baicalin of Plantago asiatica L.can stably bind to the key target AKT1.The results indicate that Plantago asiatica L.,through active components such as luteolin,baicalin,sitosterol,and dinatin,acts on key targets including AKT1,TP53,TNF,CASP3,and IL-6.This involvement in various cancer-related and PI3K-AKT signaling pathways contributes to preve
作者
韦国旺
黄甫克
胡湘云
盛双
韦良炬
韦宣亦
WEI Guowang;HUANG Fuke;HU Xiangyun;SHENG Shuang;WEI Liangju;WEI Xuanyi(Guangxi Hechi Institute of Science and Technology Information,Hechi 547000,China;Institute of Animal Husbandry,Guangxi Vocational University of Agriculture,Nanning 530007,China;Guangxi Hechi Technology Development Center,Hechi 547000,China;Guangxi Hechi Technology Market Service Center,Hechi 547000,China)
出处
《中国兽医杂志》
CAS
北大核心
2024年第3期119-125,共7页
Chinese Journal of Veterinary Medicine
基金
广西创新驱动发展专项资金项目(桂科AA19254007)
河池市本级科技计划项目(河科A1842-1-1.1)。
关键词
车前草
腹泻
网络药理学
Plantago asiatica L.
diarrhea
network pharmacology
