摘要
目的探讨葛根素对老年性耳聋以及学习记忆的干预作用。方法将小鼠随机分为葛根素组、模型组和对照组。听觉脑干反应(ABR)检测小鼠听觉功能,morris水迷宫检测小鼠学习记忆能力,免疫荧光染色激光共聚焦显微镜观察耳蜗毛细胞及带状突触形态的改变,3dsMax软件对带状突触进行三维重建并计算带状突触数量的改变。结果葛根素干预后显著降低模型小鼠ABR阈值,增加带状突触的数量。葛根素显著降低模型小鼠水迷宫逃避潜伏期,增加跨越平台的次数和目标象限探索时间。结论葛根素通过降低小鼠ABR阈值和增加带状突触的数量而改善听力功能,同时葛根素还能增强耳聋小鼠的学习和记忆能力。
Objective To investigate the effect of puerarin on age-related hearing loss(ARHL)and learning-memory ability.Methods Mice were randomly divided into puerarin group,model group and control group.Theauditory brainstem response(ABR)was used to detect the auditory function,the morris water maze was used to detect the learning and memory ability,and the changes of ribbon synapses were observed by immunofluorescence.Results After the intervention of puerarin,the ABR threshold of model mice was significantly reduced and the number of ribbon synapses was increased.Puerarin also significantly reduced the escape latency of model mice,increased the number of crossing platforms and the exploration time in the target quadrant.Conclusion Puerarin can improve hearing function by lowering the ABR threshold and increasing the number of ribbon synapses.At the same time,puerarin can also enhance the learning-memory ability of ARHL mice.
作者
张凤英
周进
隋轶
ZHANG Feng-ying;ZHOU Jin;SUI Yi(Department of Neurology,The first hospital of Shenyang city,Shenyang 110041,China)
出处
《解剖科学进展》
CAS
2023年第6期631-633,638,共4页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金(20180550945)
沈阳市科技局项目(202054071)。
