摘要
辅助生殖技术(ART)在不孕不育诊治、濒危物种保存、良种家畜快速高效扩繁中具有重要应用价值。ART已常规开展并在世界范围内应用,但该技术面临着效率低下等挑战。非生理状态下的培养和保存可致配子和胚胎处于氧化应激状态,越来越多的研究表明氧化应激是影响精子获能、损伤卵母细胞及胚胎发育能力的主要因素。近年来,创新性使用纳米颗粒(NPs)的相关技术和方案有效降低了配子氧化应激水平,提高了胚胎生物技术的效率。本文系统分析了NPs进入细胞的方式、分布及影响因素,深入探讨了NPs的作用机理,归纳NPs在配子、体外胚胎生产、低温保存中的应用,以期为ART总体效率的提高提供新方法和理论依据。
Assisted reproductive technology(ART)holds significant promise for infertility diagnosis and treatment,endangered species preservation,rapid and efficient propagation of superior varieties.Although ART has been routinely performed and applied worldwide,it also faces some challenges,such as the suboptimal efficacy of the technique.It is demonstrated that gametes and embryos are under oxidative stress during non-physiological culture and preservation,and a growing body of evidence indicates that oxidative stress is mainly accounted for the decreased sperm capacitation as well as the compromised developmental potential in oocyte and embryos.In recent years,decreased oxidative stress level in gametes as well as improved efficiency of in embryo biotechnology has been achieved by the innovative application of nanoparticle(NPs)related technologies and schemes.In the present article,the entry mode and distribution of NPs as well as the influencing factors are systematically analyzed.Moreover,the mechanism underlying the action of NPs is discussed in depth.Finally,the current application progress of NPs in gametes,in vitro embryo production and cryopreservation is summarized,with the purpose to provide a new method and theoretical basis for improving the overall efficiency of ART.
作者
海桂萍
李俊
刘倩
刘昱成
万鹏程
阿布力孜·吾斯曼
傅祥伟
HAI Guiping;LI Jun;LIU Qian;LIU Yucheng;WAN Pengcheng;ABULIZ·Wusiman;FU Xiangwei(College of Animal Science,Xinjiang Agricultural University,Xinjiang Urumqi 830052,China;Reproductive Medical Center,The First Hospital of Hebei Medical University,Hebei Shijiazhuang 050031,China;College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;State Key Laboratory of Sheep Genetic Improvement and Healthy Production,Xinjiang Shihezi 832000,China)
出处
《中国畜牧杂志》
CAS
CSCD
北大核心
2024年第2期64-70,共7页
Chinese Journal of Animal Science
基金
中央引导地方科技发展资金项目(纳米颗粒3D、226Z7713G)
兵团科技发展专项(S2021BC505)
河北省政府资助临床医学优秀人才培养项目。
关键词
纳米颗粒
配子
胚胎
体外胚胎生产
低温保存
氧化应激
Nanoparticle
Gamete
Embryo
In vitro embryo production
Cryopreservation
Oxidative stress
