摘要
目的 基于磷脂酰肌醇激酶(PI3K)/蛋白激酶B(AKT)通路探讨何氏补肾厚膜方(HBHP)改善大鼠薄型子宫内膜(TE)的作用机制。方法 48只SD雌性大鼠,按体质量随机分为对照组、模型组、阳性组和HBHP组,每组12只。除对照组外,其余各组在发情期对大鼠右侧子宫注入95%乙醇建立TE模型。HBHP组在模型建立后灌胃给药HBHP 10 g/(kg·d),按1 mL/100 g灌胃。阳性组给予小剂量阿司匹林肠溶片0.5 mg/(kg·d),按1 mL/100 g灌胃;对照组及模型组每日灌服生理盐水1 mL/100 g。各组大鼠连续灌胃15 d后,剖腹取子宫。苏木精-伊红(HE)染色观察子宫内膜厚度的变化。免疫组织化学染色和逆转录-定量实时聚合酶链反应(RT-qPCR)检测子宫内膜组织中PI3K、AKT蛋白和mRNA的表达。结果 与对照组比较,模型组大鼠的子宫内膜厚度[(357.34±14.35)μm比(526.08±23.21)μm]、腺体数量[(8.09±1.57)比(19.08±1.31)]、子宫湿重[(0.42±0.05)g比(0.80±0.06)g]和子宫脏器指数[(0.16±0.02)%比(0.30±0.03)%]显著降低(P<0.05)。与模型组比较,HBHP组、阳性组子宫内膜厚度[(476.74±21.42)μm、(438.18±22.39)μm比(357.34±14.35)μm]、腺体数量[(16.09±1.34)、(13.91±1.45)比(8.09±1.57)]、子宫湿重[(0.75±0.06)g、(0.68±0.08)g比(0.42±0.05)g]和子宫脏器指数[(0.26±0.02)%、(0.24±0.03)%比(0.16±0.02)%]显著增加(P<0.05)。与对照组比较,模型组子宫内膜组织中PI3K、AKT及p-AKT蛋白表达[PI3K:(0.15±0.01)比(0.42±0.02);AKT:(0.21±0.01)比(0.71±0.11);p-AKT:(0.37±0.02)比(0.88±0.06)]和AKT、PI3K mRNA相对表达量[AKT:(0.42±0.20)比(1.02±0.23);PI3K:(0.58±0.22)比(1.06±0.35)]显著下调(P<0.05)。与模型组比较,HBHP组、阳性组子宫内膜组织中PI3K、AKT及p-AKT蛋白表达[PI3K:(0.33±0.03)、(0.28±0.02)比(0.15±0.01);AKT:(0.57±0.03)、(0.43±0.05)比(0.21±0.01);p-AKT:(0.71±0.04)、(0.54±0.03)比(0.37±0.02)]和AKT、PI3K mRNA相对表达量[AKT:(0.97±0.33)、(0.86±0.30)比(0.42±0.20);PI3K:(0.98±0.
Objective To explore the mechanism of Heshi Bushen Houmo Prescription(HBHP)in improving thin endometrium(TE)in rats through the phosphatidylinositol kinase(PI3K)/protein kinase B(AKT)pathway.Methods Based on body weight,48 female Sprague Dawley rats were randomly divided into control,model,positive control,and HBHP groups,with 12 rats in each group.Except for the control group,a TE model was developed by injecting 95%ethanol into the right uterus of rats during estrus phase in all other groups.In the HBHP group,10 g/(kg·d)of HBHP(1 mL/100 g)was administered by gavage after establishing the model.Rats in the positive group received a small dose of enteric-coated aspirin tablets at 0.5 mg/(kg·d)(1 mL/100 g)through gavage.The control and model groups received a daily gavage of 1 mL/100 g normal saline.After 15 days of continuous gavage,the uteri were dissected by laparotomy in each group.HE staining was used to detect the changes in endometrial thickness.Immunohistochemical staining and reverse transcription-quantitative real-time polymerase chain reaction were used to detect the protein and mRNA expression of PI3K and AKT in endometrial tissues.Results Compared with the control group,the model group exhibited significant decrease in endometrium thickness(357.34±14.35 vs.526.08±23.21μm),gland count(8.09±1.57 vs.19.08±1.31),uterus wet weight(0.42±0.05 vs.0.80±0.06 g)and uterine organ index(0.16±0.02 vs.0.30±0.03%,P<0.05).Compared with the model group,the HBHP and positive groups showed significant increases in endometrium thickness(476.74±21.42,438.18±22.39 vs.357.34±14.35μm),gland count(16.09±1.34,13.91±1.45 vs.8.09±1.57),the uterus wet weight(0.75±0.06,0.68±0.08 vs.0.42±0.05 g)and uterine organ index(0.26±0.02,0.24±0.03 vs.0.16±0.02%,P<0.05).Compared with the control group,the model group exhibited significant decrease in the protein expression of PI3K(0.15±0.01 vs.0.42±0.02),AKT(0.21±0.01 vs.0.71±0.11)and p-AKT(0.37±0.02 vs.0.88±0.06),as well as the relative mRNA expression of AKT(0.4
作者
徐新亚
陈碧霞
吴晓婷
XU Xin-ya;CHEN Bi-xia;WU Xiao-ting(Department of Traditional Chinese Medicine Gynaecology,Hangzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University,Hangzhou,Zhejiang 310007,China)
出处
《浙江中西医结合杂志》
2024年第3期214-218,共5页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省基础公益研究计划项目(No.LGF20H270008)。
