摘要
Over the past decade,advances in single-cell omics(SCO)technologies have enabled the investigation of cellular heterogeneity at an unprecedented resolution and scale,opening a new avenue for understanding human biology and disease.In this review,we summarize the developments of sequencing-based SCO technologies and computational methods,and focus on considerable insights acquired from SCO sequencing studies to understand normal and diseased properties,with a particular emphasis on cancer research.We also discuss the technological improvements of SCO and its possible contribution to fundamental research of the human,as well as its great potential in clinical diagnoses and personalized therapies of human disease.
基金
supported by the National Natural Science Foundation of China(Grant Nos.81988101,31991171,91959000,62203019,92159305,and 92259205)
the Beijing Municipal Science and Technology Commission(Grant No.Z221100007022002)
the Changping Laboratory,China.Qiang Shi was supported in part by the China Postdoctoral Science Foundation(Grant Nos.2021TQ0012 and 2022M720246)
the Peking University Boya Postdoctoral Fellowship,and the Postdoctoral Fellowship of Peking-Tsinghua Center for Life Sciences,China.
