摘要
发光的金属纳米簇由于毒性小、成本低和生物相容性好等优势引起了人们的广泛关注,然而制备具有强发光的铜纳米簇聚集体是一个很大的挑战。以手性氨基酸D-青霉胺为原料,“一步法”合成了具有强发光的铜纳米簇聚集体,并通过红外光谱、热重分析、X-射线光电子能谱、X-射线衍射、质谱、扫描电镜、透射电镜、发光光谱和激光共聚焦显微镜表征技术对其进行了详细研究。结果表明:铜纳米簇聚集体的悬浊液和固体显示出很强的发光,绝对发光量子产率高达30.4%。而且,发光的铜纳米簇聚集体细胞毒性很低,可以在人肝癌细胞HepG2中实现胞内成像以及依赖于温度的胞内成像。铜纳米簇聚集体的发光行为在细胞成像方面的应用探索为人们在其它领域进行应用提供了重要参考。
Emissive metal nanoclusters have received considerable research attention owing to their low toxicity,low cost,and good biocompatibility,but the preparation of Cu nanoclusters assemblies with strong emission behavior is a large challenge.Chiral ligand D-penicillamine was used as a reactant to construct intense emission of luminescent Cu nanoclusters assembly by“one-pot”method.They have been characterized by multiple techniques including FT-IR specta,thermogravimetric analysis,X-ray photoelectron spectra,X-ray diffraction pattern,scanning electron microscopy(SEM),transmission electron microscopy(TEM),photoluminescence spectra,and cellular imaging.The results show that Cu nanoclusters assemblies in suspension solution and solid state show very strong emission with the absolute photoluminescence quantum yield as high as 30.4%.Moreover,emissive Cu nanoclusters assemblies possess very low cellular toxicity and can be used as cellular imaging as well as the temperature-dependent intracellular imaging agent in HepG2 cells.The application exploration on cellular imaging of Cu nanoclusters assembly with strong emission behavior provides significant references for their applications in other areas.
作者
孔令灿
KONG Lingcan(The Affiliated Wuxi Center for Disease Control and Prevention of Nanjing Medical University,Wuxi 214023,China)
出处
《合成化学》
CAS
2024年第3期237-243,共7页
Chinese Journal of Synthetic Chemistry
基金
国家自然科学基金资助项目(22075106)
无锡市医学创新团队项目(CXTD2021004)。
关键词
铜纳米簇聚集体
自组装
纳米合成
发光
细胞成像
温度传感
Cu nanoclusters assembly
self-assemble
nano synthesis
emission
cellular imaging
temperature sensing
