摘要
为探明经简易加工后的园林废弃物用于地表覆盖对土壤细菌群落结构及功能特征的影响.本研究将园林废弃物系统分为4种组分材料(草屑、树叶、枝叶、树枝)进行试验,设置3个覆盖厚度(3 cm、6 cm、9 cm),并以无覆盖的裸土为对照.利用16S rDNA高通量测序技术,分析经过21个月覆盖的土壤细菌群落结构及功能差异.研究结果显示,覆盖处理显著影响土壤细菌群落结构,但对细菌多样性无显著影响.土壤细菌优势门为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和放线菌门(Actinobacteria).土壤细菌优势属受覆盖的影响表现不一,树枝和3 cm厚度草屑处理的优势细菌是伯克霍尔德菌属(Burkholderia),6 cm厚度草屑处理的优势细菌是Candidaius_Koribacter,对照及其余处理的优势细菌是Kaistobacter,它们均属于土壤有益细菌.冗余分析表明,土壤有机质、全氮、碱解氮、全磷、有效磷、全钾是细菌群落结构的关键影响因子.功能预测结果显示,草屑、3 cm和6 cm厚度树叶、9 cm厚度枝叶处理的二级功能层结构较对照相对稳定;树枝、9 cm厚度树叶、3 cm和6cm厚度枝叶处理提高了膜运输、辅助因子和维生素的代谢、萜类和聚酮化合物的代谢等功能基因丰度.总体而言,园林废弃物地表覆盖通过改变土壤细菌群落结构,进而调控细菌群落代谢等功能基因的表达;从生态效益出发,树枝、9 cm厚度树叶、3 cm和6 cm厚度枝叶覆盖具有提升土壤物质循环与转化能力的潜力,是园林废弃物地表覆盖应用的优势处理.
This study aims to explore the impact of simple processing of green waste mulches on the structure and functional characteristics of soil bacterial community.In this study,the green waste was classified into four groups;grass clippings(TCS),leaves(LVS),a mixture of branches and leaves(MBL),and branches(BCS),and three mulching thicknesses(3 cm,6 cm,9 cm)were set up.Employing 16S rDNA high-throughput sequencing technology,the structural and functional dynamics of soil bacterial communities after 21 months of mulching was analyzed.The results showed that mulching significantly affected soil bacterial community structure,but had no significant influence on bacterial diversity.The dominant phyla of soil bacteria observed were Proteobacteria,Acidobacteria and Actinobacteria.The mulching influenced dominant bacterial genera,i.e.,Burkholderia in BCS and TCS(3 cm),Candidatus_Koribacter in TCS(6 cm),and Kaistobacter in the control and other treatments.Redundancy analysis results showed that soil organic matter,total nitrogen,available nitrogen,total phosphorus,available phosphorus and total potassium were the key influencing factors of bacterial community structure.Functional predictions highlighted relatively stable hierarchy levels in TCS,LVS(3 cm,6 cm)and MBL(9 cm)compared to the control.BCS,LVS(9 cm)and MBL(3 cm,6 cm)treatments increased the abundance of functional genes such as membrane transport,metabolism of cofactors and vitamins,and metabolism of terpenoids and polyketides.In general,the green waste mulches influenced the structure of soil bacterial community,regulating the expression of functional genes and metabolism.From the perspective of ecological benefits,treatments with BCS,LVS(9 cm)and MBL(3 cm,6 cm)have the potential to improve the recycling and transformation capacity of soil material,which were benefical for the application of green waste mulches.
作者
潘松
史正军
毛晓宁
董志强
袁峰均
罗越
PAN Song;SHI Zhengjun;MAO Xiaoning;DONG Zhiqiang;YUAN Fengjun;LUO Yue(Key Laboratory of Southern Subtropical Plant Diversity,Fairy Lake Botanical Garden,Shenzhen&Chinese Academy of Sciences,Shenzhen 518004;China Shenzhen Urban Forest Ecosystem Research Station,State Administration of Forest and Grassland,Shenzhen 518004;College of Life Sciences,Qinghai Normal University,Xining 810008)
出处
《环境科学学报》
CAS
CSCD
北大核心
2024年第2期386-395,共10页
Acta Scientiae Circumstantiae
基金
国家重点研发计划(No.2021YFE0193200)
深圳市科技计划项目(No.KCXFZ202002011006491,JSGG20170824090848015)
深圳市城管和综合执法局科研项目(No.202004,202018,202201)。
关键词
园林废弃物
覆盖
细菌群落
高通量测序
功能预测
green waste
mulch
bacterial community
high-throughput sequencing
function prediction
