摘要
在单因素试验的基础上,采用L9(34)正交试验,以小扁豆萌发前后总黄酮类物质提取率为指标,探讨了提取温度、乙醇浓度、料液比、提取次数对总黄酮类物质提取率的影响。在优化小扁豆中总黄酮类物质提取工艺的基础上,对比了萌发前后黄酮类物质的含量变化。结果表明,小扁豆萌发前最佳提取工艺参数为:提取温度85℃、乙醇体积分数60%、料液比1∶60、回流8h提取2次;萌发后最佳提取工艺参数为:提取温度90℃、乙醇体积分数60%、料液比1∶20、回流8h提取2次。在最佳工艺条件下,测得萌发前小扁豆中总黄酮类物质的平均含量为10.452mg/g,萌发后为8.518mg/g。该方法操作简单,稳定性和重复性好,本实验首次测定了萌发前后小扁豆中总黄酮类物质含量,为其深加工提供了依据。
On the basis of single factor experiment,L9(34)orthogonal test was used to study the effects of extraction temperature,ethanol concentration,solid-liquid ratio and extraction times on the extraction rate of total flavonoids from lentils in pre-and post-germination.On the basis of optimizing the extraction process of total flavonoids in lentils,the content changes of flavonoids in lentils at pre-and post-germination stage were compared.The results showed that the optimumextraction temperature 85℃,ethanol volume fraction 60%,material to liquid ratio 1∶60(g/ml),refluxing extraction for twice with 8h every time;the optimum extraction process parameters after germination were as follows:extraction temperature 90℃,ethanol volume fraction 60%,material to liquid ratio 1∶20(g/ml),refluxing extraction for twice with 8h every time.Under the optimum conditions,the average content of total flavonoids in lentils was 10.452 mg/g before germination and 8.518 mg/g after germination.The extraction method is simple,stable and reproducible.In this experiment,the content of total flavonoids in lentils at pre-and post-germination stage was determined for the first time,which provided a basis for its deep processing.
作者
贾海燕
赵瑶
拓明娜
JIA Hai-yan;ZHAO Yao;TUO Ming-na(College of Life Science and Technology,Longdong University,Qingyang 745000,Gansu;Provincial Key Laboratory for Protection and Utilization of Longdong Bio-resources in Gansu,Qingyang 745000,Gansu)
出处
《陇东学院学报》
2024年第2期107-111,共5页
Journal of Longdong University
基金
甘肃高等学校科学研究项目(2021A-124)
陇东学院青年科技创新基金项目(XYZK2304)。
关键词
小扁豆
黄酮类物质
单因素
正交实验
Lens culinaris
flavonoids
single factor test
orthogonal test
