摘要
目的 研究D-阿洛糖对小鼠脑缺血再灌注损伤(IRI)导致的星形胶质细胞细胞焦亡的保护作用。方法 构建大脑中动脉缺血栓塞模型模拟小鼠脑IRI,经腹腔注射D-阿洛糖治疗(400mg·kg^(-1)),神经功能评分以及行为学实验检测其大脑损伤情况,苏木精-伊红染色观察大脑组织病理结构改变,TUNEL染色检测细胞凋亡情况,蛋白印迹检测焦亡相关蛋白消皮素D (GSDMD)和白细胞介素-18 (IL-18)的表达水平,免疫组化与免疫荧光实验检测星形胶质细胞中的焦亡相关蛋白(GSDMD、IL-18)的表达情况。结果 相较于IRI组,经D-阿洛糖治疗后改善了神经功能评分以及行为学表现,减轻了因IRI导致的脑形态学改变,在大脑皮质中焦亡相关蛋白(GSDMD、IL-18)表达降低,并且减少了在星形胶质细胞中焦亡相关蛋白(GSDMD)的表达。结论 在小鼠脑IRI模型中,D-阿洛糖可以减轻星形胶质细胞的焦亡,从而起到保护大脑神经元的作用。
Objective To study the protective effect of D-allose on pyroptosis induced by ischemiareperfusion injury(IRI)in mice brain astrocytes.Methods The middle cerebral artery occlusion model was constructed to simulate cerebral ischemia-reperfusion injury in mice.D-allose was injected intraperitoneally(400mg·kg^(-1)),and the neurological function was evaluated to detect the brain injury.Pathological changes of brain tissues were observed by hematoxylin-eosin staining,and cell apoptosis was detected by TUNEL staining.Western blot was used to detect the expression of pyroptosis related proteins(GSDMD,IL-18),and immunohistochemistry and Immunofluorescence technique were used to detect the expression of pyroptosis related proteins(GSDMD,IL-18)in astrocytes.Results Compared with ischemia-reperfusion group,treating with the D-allose improved the neurological function and behavior performance,reduced the morphology change caused by ischemia-reperfusion injury.Pyroptosis related proteins(GSDMD,IL-18)in the brain cortex expression were reduced,and decreased the expression of pyroptosis related proteins(GSDMD)in astrocytes.Conclusion In the model of cerebral ischemia-reperfusion injury,D-allose can reduce pyroptosis of astrocytes and make a neuroprotective role.
作者
付奕豪
张敏
罗耀文
张磊
高大宽
Fu Yihao;Zhang Min;Luo Yaowen;Zhang Lei;Gao Dakuan(Department of Neurosurgery,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)
出处
《脑与神经疾病杂志》
CAS
2024年第3期157-161,共5页
Journal of Brain and Nervous Diseases
基金
国家自然科学基金面上项目(81971227)
陕西省社会发展科技攻关项目-重点项目(2018ZDXM-SF-086)。
关键词
D-阿洛糖
缺血再灌注损伤
焦亡
器官保护
D-allose
Ischemia/reperfusion injury
Pyroptosis
Organ protection
