摘要
为建立陇东黑山羊胸膜肺炎病原的分离与鉴定方法,选取甘肃省陇东地区40例黑山羊胸膜肺炎疑似病例作为研究对象,通过病原菌分离培养、染色镜检等方法进行鉴定。共分离培养出6株典型的单克隆菌株,镜检可见病原菌呈球形、短杆状、长杆状或多形状。生化试验显示,除MC4菌株的四氮唑还原试验呈阴性外,其余生化试验结果均与标准菌株PG3的结果一致。血清凝集试验表明,病羊血清和阳性血清与抗原可发生凝集反应。分离的6株病原菌经PCR检测均可扩增出一大小为423 bp的特异性电泳条带。本研究成功建立陇东黑山羊胸膜肺炎的分离与鉴定方法,为遏制本病的蔓延扩散和精准防控提供参考依据。
To establish a method for isolation and identification of the pathogen of pleuropneumonia in Longdong black goats.40 suspected cases of pleuropneumonia in black goats from the Longdong area of Gansu Province were selected as the research subjects,and identified through methods such as pathogen isolation and cultivation,staining microscopy.As a result,a total of 6 typical monoclonal strains were isolated and cultured,and microscopic examination showed that the pathogenic bacteria were spherical,short rod-shaped,long rod-shaped,or multi shaped.The biochemical tests showed that except for the negative tetrazolium reduction test of MC4 strain,the results of all other biochemical tests were consistent with those of the standard strain PG3.The serum agglutination test shows that diseased sheep serum and positive serum can undergo agglutination reaction with antigens.Six isolated pathogenic bacteria were detected by PCR and all amplified with a specific electrophoresis band of 423 bp in size.This study successfully established a method for isolation and identification of pleuropneumonia in Longdong black goats,which provides a reference basis for controlling the spread and precise prevention of this disease.
作者
李玉洁
蒋东平
李嘉龙
王胜林
张武浩
张元
张海军
王珂
LI Yujie;JIANG Dongping;LI Jialong;WANG Shenglin;ZHANG Wuhao;ZHANG Yuan;ZHANG Haijun;WANG Ke(Gansu Provincial Institute of Animal Husbandry and Veterinary Medicine,Pingliang Gansu 744000,China;Huachi County Animal Husbandry and Veterinary Station,Qingyang Gansu 745600,China;Agricultural and Rural Comprehensive Service Center in Ruoyuan Town,Huachi County,Qingyang Gansu 745600,China;Huachi County Animal Disease Prevention and Control Center,Qingyang Gansu 745600,China)
出处
《现代畜牧科技》
2024年第2期9-11,共3页
Modern Animal Husbandry Science & Technology
基金
“陇原之光”人才培养计划(甘组通字[2022]198号)。
关键词
黑山羊
胸膜肺炎
分离
鉴定
black goat
pleural pneumonia
separation
appraisal
