摘要
目的考察弩药微乳多成分在自制探针上的回收率与温度、灌流速度、浓度的关系,研究弩药微乳多成分在家兔皮肤组织中的药动学特征。方法采用UPLC测定绿原酸、苯甲酰新乌头原碱、杠柳毒苷、新乌头碱质量浓度;采用增量法与减量法探究温度、灌流速度、质量浓度对探针回收率的影响,UPLC测定弩药微乳经皮给药后不同时间点透析液中多成分含量变化,并用DAS2.0软件处理数据。结果升高温度,各成分探针回收率逐渐提高;流速越大,药物回收率越低,且回收率与探针周围药物质量浓度关系不大。弩药微乳中绿原酸、苯甲酰新乌头原碱、杠柳毒苷、新乌头碱在皮下组织中的药动学参数C max分别为(3.24±0.36)(1.04±0.29)(4.78±2.15)(2.86±0.55)μg·mL^(-1);AUC 0-∞分别为(52.42±30.84)(7.70±3.73)(42.23±13.93)(22.68±12.51)h·μg·mL^(-1)。结论首次采用微透析在体实时取样技术,建立UPLC检测方法,对弩药微乳经皮给药后多成分皮肤药动学进行研究,为弩药微乳后续的考察及应用提供参考。
OBJECTIVE To investigate the pharmacokinetic characteristics of crossbow microemulsion in rabbit skin.METHODS The concentrations of chlorogenic acid,benzoyl new aconitine,periplocin and new aconitine were determined by UPLC.The effects of temperature,perfusion speed and mass concentration on the recovery rate of the probe were investigated by incremental and decrement methods,the changes of multi-component content in the dialysate at different time points after transdermal administration of crossbow microemulsion were determined by UPLC,and DAS 2.0 software was used to process the data.RESULTS The recovery rate of the probe increased with increasing temperature,and the higher the flow rate,the lower the recovery rate.The pharmacokinetic parameters of chlorogenic acid,benzoyl new aconitine,periplocin and new aconitine in microemulsion C max were(3.24±0.39)(1.04±0.29)(4.78±2.15)(2.86±0.55)μg·mL^(-1),respectively,and in AUC 0-∞were(52.42±30.84)(7.70±3.73)(42.23±13.93)(22.68±12.51)h·mg·mL^(-1),respectively.CONCLUSION A real-time in vivo microdialysis sampling technique was first used and a UPLC detection technique was established to study the skin pharmacokinetics of multi-components of crossbow microemulsion after transdermal administration,which provides a reference for the follow-up investigation and application of crossbow microemulsion.
作者
谢欢
宋选飞
周斌
刘耀
杨芳芳
陈迎龙
朱艳
张永萍
XIE Huan;SONG Xuanfei;ZHOU Bin;LIU Yao;YANG Fangfang;CHEN Yinglong;ZHU Yan;ZHANG Yongping(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Institute of Engineering and Technology for Processing and Preparation of Traditional Chinese Medicine and Ethnic Medicine,Guiyang 550025,China;National Engineering and Technology Research Center of Miao Medicine,Guiyang 550025,China;College of Acupuncture and Tuina,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;School of Traditional Chinese Medicine,Guizhou Health Vocation College,Tongren 554300,China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2024年第2期167-173,共7页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(82160753)
贵州省高等学校中药民族药(苗药)新剂型新制剂工程研究中心项目(黔教技〔2022〕022)
国家苗药工程技术研究中心能力提升项目(黔科合中引地〔2023〕006)。
关键词
弩药微乳
微透析
UPLC
皮肤药动学
crossbow microemulsion
microdialysis
UPLC
skin pharmacokinetics
