摘要
生物蜡是一种新型固定化生物膜技术,其能够通过富集水体土著微生物,有效提高水体自净能力。系统地考察了生物蜡对典型江南园林水体春夏季水质的净化效能。结果表明,每100 m2水面投放1块生物蜡,能够使水体在春夏季的COD、氨氮、总氮、总磷和叶绿素a均值分别降低24.12%、42.11%、47.25%、23.53%和17.90%,透明度提高24.39%,COD、总氮、氨氮和总磷的Ⅳ类水达标率分别提高154.55%、71.43%、11.11%和93.75%。通过采用高通量测序和功能基因预测技术对水体净化的微生物反应机制进行分析,发现生物蜡表面生物膜中约82.4%的OTU来自于水体和沉积物中的土著微生物,尽管生物膜的微生物丰度和多样性指数均低于沉积物,但具备有机物和氮素降解能力的变形菌门(Proteobacteria,相对丰度69.36%)占据主导地位,并形成了复杂的微生物共现网络,增强了光能自养、化能异养和亚硝酸盐去除等代谢功能。该研究结果表明,生物蜡技术为土著功能菌群的选择性富集提供了良好载体,在园林水体原位净化领域具有广阔的应用前景。
Bio-wax is a novel immobilized biofilm technology,which can effectively improve the selfpurification ability of water bodies by enriching indigenous microorganisms.This study systematically investigated the purification effect of bio-wax on spring and summer water quality in typical Jiangnan garden water bodies.The results showed that adding one piece of bio-wax in 100 m?water surface area can reduce the mean concentration of COD,ammonia nitrogen,total nitrogen,total phosphorus and chlorophyll a by 24.12%,42.11%,47.25%,and 17.90%,respectively.It can also increase the transparency of water body by 24.39%and increase the Class IV water compliance rate of COD,total nitrogen,ammonia nitrogen and total phosphorus by 154.55%,71.43%,11.11%and 93.75%,respectively.By using high-throughput sequencing and functional gene prediction techniques,this study found that about 82.4%OTUs in the surface biofim of the bio-wax were derived from indigenous microorganisms in water bodies and sediments.It is also found that although the microbial abundance and diversity indices of the biofilm were lower than those of the sediments,Proteobacteria(relative abundance 69.36%),which have the ability of organic matter and nitrogen degradation,dominated the biofilm,and formed a complex microbial co-occurrence network with significantly enhanced metabolic functions of photoenergetic autotrophs,chemoenergetic heterotrophs,and nitrate nitrogen respiration.In conclusion,the bio-wax technology provides a good carrier for the selective enrichment of indigenous functional flora,and has a broad application prospect in the field of in situ purification of garden water.
作者
吕凌霄
张明阳
钱飞跃
刘锋
丁学瑞
李勇
李锋
LYU Lingxiao;ZHANG Mingyang;QIAN Feiyue;LIU Feng;DING Xuerui;LI Yong;LI Feng(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology,Suzhou 215009,China;Suzhou Hanrun Environmental Energy Technology Group Co.,Suzhou 215009,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2023年第12期4107-4115,共9页
Chinese Journal of Environmental Engineering
基金
江苏省自然科学基金资助项目(BK20211339)
苏州市社会发展科技创新项目(2022SS10)
苏州市建设科技项目(SZJ202129503)。
关键词
园林水体
生物蜡技术
水质净化
细菌群落
功能基因
garden water body
bio-wax technology
water purification
bacterial community
functional gene prediction
