摘要
【目的】探究嫁接对番茄植株根际微生物群落组成的影响,旨在揭示嫁接提高番茄植株抗性的机制并为构建解决番茄连作障碍体系提供参考。【方法】基于高通量测序技术,以番茄品种新砧1号和宝砧1号为砧木,桃太郎品种为接穗进行以下处理:新砧1号实生植株(简称:新砧);新砧1号×桃太郎嫁接植株(简称:桃太郎/新砧);宝砧1号实生植株(简称:宝砧);宝砧1号×桃太郎嫁接植株(简称:桃太郎/宝砧);桃太郎实生植株(简称:桃太郎)。采集番茄实生与嫁接处理植株根际土壤,比较分析番茄各处理根际土壤细菌和真菌的群落组成。【结果】与新砧、宝砧、桃太郎处理植株相比,桃太郎/新砧和桃太郎/宝砧处理植株细菌和真菌群落在不同分类水平上可操作分类单元(OTU)数量均发生明显变化。与砧木和接穗实生植株相比,番茄嫁接植株根际土壤中拥有更丰富的微生物OTU和特有OTU数量。番茄实生与嫁接植株间在根际土壤细菌和真菌多样性与丰富度上无显著差异(P>0.05)。在门分类水平上,在桃太郎/新砧处理植株根际土壤中,放线菌门和厚壁菌门细菌丰度占比均高于新砧和桃太郎处理植株;在桃太郎/宝砧处理植株中,绿弯菌门、酸杆菌门、芽单胞菌门细菌丰度占比均高于宝砧和桃太郎处理植株。在属分类水平上,与新砧和桃太郎处理植株相比,桃太郎/新砧处理植株根际土壤中鞘氨醇单胞菌属、芽孢杆菌属、链霉菌属、unclassified_f__Micro⁃coccaceae、norank_f__norank_o__Gaiellales细菌属丰度占比增加;与桃太郎处理植株相比,桃太郎/新砧处理植株根际土壤中增加特有优势菌属栖大理石菌属。与宝砧和桃太郎处理植株相比,桃太郎/宝砧处理植株根际土壤中,norank_f__norank_o__norank_c__Subgroup_6、类诺卡氏菌、norank_f__norank_o__norank_c__Gitt⁃GS⁃136、norank_f__norank_o__norank_c__KD4⁃96、norank_f__JG30⁃KF⁃CM45属�
【Objective】The aim of the study was to explore the effects of grafting on the rhizosphere microbial community of tomato plants,in order to reveal the mechanism of enhancing resistance of tomato plants by grafting,and to provide reference for constructing a system to solve obstacle of tomato continuous cropping.【Method】Based on Misseq high⁃throughput sequencing technology,Xinzhen 1 and Baozhen 1 culti⁃vars were used as rootstocks,and Taotailang cultivar was used as scion.Xinzhen 1 plant(abbreviated as Xinzhen);Xinzhen 1×Taotailang grafted plant(abbreviated as Taotailang/Xinzhen);Baozhen 1 plant(abbreviated as Baozhen 1);Baozhen 1×Taotailang grafted plant(abbreviated as Taotailang/Baozhen 1);Taotailang plant(abbreviated as Taotailang).【Result】Compared with Xinzhen,Baozhen and Tao⁃tailang plants,the number of operational taxonomic unit(OTU)of the bacterial and fungal communities in Taotailang/Xinzhen and Tao⁃tailang/Baozhen changed greatly.Compared with rootstock and scion plants,the rhizosphere soil of tomato grafted plants had more abundant microbial OTU and specific OTU.There were no significant differences in the diversity and richness of bacteria and fungi in rhizosphere soil between tomato plants and grafted plants(P>0.05).At the phylum classification level,the abundance of Actinobacteria and Firmicutes in rhizosphere soil of Taotailang/Xinzhen plants was higher than that of Xinzhen and Taotailang plants.The bacterial abundance of Chloroflexi,Acidobacteria and Gemmatumonadetes in Taotailang/Baozhen plants were higher than Baozhen and Taotailang plants.At the genus level,compared with the Xinzhen and Taotailang plants,the abundance of Sphingomonas,Bacillus,Streptomyces,unclassified_f__Micrococcaceae and norank_f__norank_o__Gaiellales in rhizosphere soil of Taotailang/Xinzhen plants increased.Compared with Taotailang plants,endemic dominant bacteria(Marmoricola)were found in rhizosphere soil of Taotailang/Xinzhen plants.Compared with the Banzhen and Taotailang plants,in the rhizosphere soil
作者
朱瑜
周信雁
魏宇飞
谢源源
韦勋
杨尚东
庞师婵
ZHU Yu;ZHOU Xin-yan;WEI Yu-fei;XIE Yuan-yuan;WEI Xun;YANG Shang-dong;PANG Shi-chan(College of Agriculture,Guangxi University/Guangxi Key Laboratory of Agricultural Products Safety/National Experimental Teaching Dem-onstration Center of Plant Science,Nanning 530004,China;Guangxi South of Tropical Agricultural Science,Longzhou,Guangxi 532415,China)
出处
《西南农业学报》
CSCD
北大核心
2024年第1期199-209,共11页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31860547)
广西重点研发计划项目(桂科AB23026076)。
关键词
番茄
嫁接
根际
微生物群落组成
Tomato
Grafting
Rhizosphere
Microbial community compositions
