摘要
目的为了研究纳米Fe_(3)O_(4)在微生物降解煤产甲烷(CH_(4))过程中的催化作用,方法采用晋城矿区矿井水和中原油田土壤中的两种菌群(厌氧消化菌,义马矿区)的煤样,考查温度和纳米Fe_(3)O_(4)添加量对反应体系产CH_(4)摩尔浓度的影响,分析产CH_(4)过程中伴随气体CO_(2)和H_(2)的摩尔浓度变化及其对CH_(4)摩尔浓度的影响,评估厌氧消化产CH_(4)反应体系中铁的作用和最大负载量。结果通过分析厌氧性产CH_(4)古菌和多环芳烃分解菌共营降解煤的生物化学过程,认为煤降解产CH_(4)厌氧反应由两个主要步骤组成:一是稠环芳烃活化的羧化反应,该反应使煤被多环芳烃分解菌降解为甲苯酚和2-萘甲酸等化合物;二是产甲烷古菌利用煤分解产生的有机酸、甲基和气体等,通过辅酶的生物化学反应生成CH_(4),该过程CH_(4)产气量随温度升高而增大,起始反应需要大量CO_(2)气体。在产甲烷古菌和多环芳烃分解菌共营系统中添加纳米Fe_(3)O_(4)后,产CH_(4)量增加了39.6%~50.8%。纳米Fe_(3)O_(4)对生物化学过程的催化作用表现在促进中间产物(H_(2)和CO_(2)等)的生成,同时生物化学反应通过消耗H_(2)和含氧有机物并生成液态水以降低密闭实验系统的气体总压力,使催化反应持续正向进行。结论纳米Fe_(3)O_(4)对微生物降解煤产CH_(4)的催化作用需要多菌种互营环境,催化过程中存在最佳负载量。
Objective To study the catalytic effect of nano-Fe_(3)O_(4) on producing methane(CH_(4))in the process of microbial degradation of coal,Methods two types of microbial communities,which were come from the mine water from Jincheng mining area and soil from Zhongyuan oilfield,were used to anaerobic digest coal samples from Yima mining area.The effects of temperature and nano Fe_(3)O_(4) addition quantity on the molar concentration of CH_(4) produced in the reaction system were investigated.The changes of the molar concentrations of CO_(2) and H_(2) were also analyzed during the process of CH_(4) production;their effects on the molar concentration of CH_(4) were evaluated.The role and maximum loading of iron were also evaluated in the anaerobic digestion reaction system and CH_(4) production.Results Basing on analysis of co-cultivation results of anaerobic methanogenic archaea and polycyclic aromatic hydrocarbon decomposing bacteria;it was believed that the anaerobic reaction of coal degradation and methanogenesis were consisting of two main steps,the first step was the carboxylation reaction of fused-ring aromatic hydrocarbon activation,which degraded coal into pmethylphenol and 2-naphthalenecarboxylic acid-;the second step was that organic acids,methyl groups and gases produced by coal decomposition were used to generate methane through the biochemical reaction of coenzymes,the yield of methane in this process increased with the increasing of temperature,and a large amount of CO_(2) had been existed in the initiating reaction of the process.The production of methane gas increased 39.6%~50.8%after adding nano-Fe_(3)O_(4) into the bacteria co-cultivation system.The catalytic effect of nano-Fe_(3)O_(4) on the process of production of methane gas was to promote the production of intermediate products(hydrogen,CO_(2) etc.),which were consumed by methanogenic archaea and liquid water was generated to reduce the gas pressure of the reaction system,so the catalytic reaction could continue in a positive direction.Conclusion
作者
赵发军
邓奇根
ZHAO Fajun;DENG Qigen(College of Safety Science and Engineering,Henan Polytechnic University,Jiaozuo 454000,Henan,China;State Key Laboratory Cultivation Base for Gas Geology and Gas Control,Henan Polytechnic University,Jiaozuo 454000,Henan,China;Collaborative Innovation Center of Coal Work Safety,Henan Province,Henan Polytechnic University,Jiaozuo 454000,Henan,China)
出处
《河南理工大学学报（自然科学版）》
CAS
北大核心
2024年第1期42-50,共9页
Journal of Henan Polytechnic University（Natural Science）
基金
国家自然科学基金资助项目(51404099)
河南省科技攻关计划项目(182102310828)。
