摘要
【目的】探究富含谷胱甘肽的酵母水解物对干露胁迫下的凡纳滨对虾(Litopenaeus vannamei)肝胰腺糖代谢相关基因的影响,为谷胱甘肽酵母水解物在水产养殖中的应用提供参考。【方法】在基础饲料(鱼粉质量分数17.7%)中分别按质量分数0%、0.3%、0.5%、0.7%、0.9%、1.1%的比例添加谷胱甘肽酵母水解物,配制等氮等脂饲料6组(分别记为G0、G0.3、G0.5、G0.7、G0.9、G1.1),饲喂初始体质量(0.24±0.01)g凡纳滨对虾4周,测定对虾生长性能指标;对对虾进行干露胁迫30 min,测定对虾肝胰腺相关基因的相对表达。【结果】各处理组对虾增重率、特定生长率和饲料系数无显著差异(P>0.05)。干露胁迫后,G0组凡纳滨对虾肝胰腺缺氧诱导因子(HIF-1α)、果糖磷酸激酶(PFK)和乳酸脱氢酶(LDH)基因的表达显著上调(P<0.05),琥珀酸脱氢酶(SDH)、苹果酸脱氢酶(MDH)和细胞色素氧化酶(CCO)基因显著下调(P<0.05),葡萄糖转运蛋白1(GLUT1)、己糖激酶(HK)和丙酮酸激酶(PK)基因的表达无显著变化(P>0.05)。饲喂含谷胱甘肽酵母水解物处理组的凡纳滨对虾肝胰腺HIF-1α、GLUT1、HK、PFK、PK和LDH基因的表达未因干露胁迫而发生显著变化(P>0.05)。G0.5和G0.7组的凡纳滨对虾肝胰腺SDH、MDH和CCO基因的表达显著均高于G0组(P<0.05)。【结论】饲料中添加谷胱甘肽酵母水解物不影响凡纳滨对虾的生长;干露胁迫使凡纳滨对虾有氧呼吸减弱,无氧呼吸增强,添加适量谷胱甘肽酵母水解物可使其仍维持较强的有氧呼吸代谢,提高凡纳滨对虾抗干露胁迫的能力。
【Objective】The aim of this paper is to investigate the effect of glutathione-rich yeast hydrolysate on genes related to hepatopancreatic glucose metabolism of Litopenaeus vannamei under air exposure,and to provide a reference for the application of glutathione-enriched yeast hydrolysate in aquaculture.【Method】The basic diet(fish meal mass fraction 17.7%)was supplemented with glutathione yeast hydrolysate at mass fraction of 0%,0.3%,0.5%,0.7%,0.9%,1.1%(named G0,G0.3,G0.5,G0.7,G0.9,G1.1,respectively)to prepare six isonitrogenous and isolipid diets.The shrimps with initial weight(0.24±0.01)g were fed with the diets respectively for 4 weeks and then the index of growth performance of the shrimps were determined.The shrimps were subjected to air exposure for 30 min.The relative expression of hepatopancreas glucose metabolism related genes were determined.【Results】The changes of weight gain rate,specific growth rate and feed conversion rate in all treatment groups were not significant(P>0.05).The expression of hypoxia inducible factor 1α(HIF 1α),phosphofructokinase(PFK),and lactate dehydrogenase(LDH)genes in hepatopancreas of the G0 group were significantly up-regulated(P<0.05),but the expression of succinate dehydrogenase(SDH),malate dehydrogenase(MDH)and cytochrome oxidase(CCO)genes in hepatopancreas of the G0 group was significantly down-regulated after air exposure(P<0.05).The expressions of glucose transporter 1(GLUT1),hexokinase(HK),and pyruvate kinase(PK)genes in hepatopancreas of the G0 group did not change significantly after air exposure(P>0.05).The expression of HIF-1α,GLUT1,HK,PFK,PK,and LDH genes in hepatopancreas of the same treatment did not change significantly due to air exposure(P>0.05).The expressions of hepatopancreas SDH,MDH,and CCO genes in the G0.5 and G0.7 groups were significantly higher than in the G0 group after air exposure(P<0.05).【Conclusion】Adding glutathione yeast hydrolysate to diets did not affect the growth of L.vannamei.Air exposure weakened aerobic respiration an
作者
陈建东
张玲
程涛
胡祥娜
杨凡
易建华
杨志龙
李兆文
谭北平
迟淑艳
曹爱巧
CHEN Jiandong;ZHANG Ling;CHENG Tao;HU Xiangna;YANG Fan;YI Jianhua;YANG Zhilong;LI Zhaowen;TAN Beiping;CHI Shuyan;CAO Aiqiao(Guangdong Ocean University,Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Zhanjiang 524088,China;Shenzhen Institute of Quality&Safety Inspection and Research,Shenzhen 518055,China;Angel Yeast Co.,Ltd,Hubei Provincial Key Laboratory of Yeast Function,Yichang 443003,China)
出处
《广东海洋大学学报》
CAS
CSCD
北大核心
2024年第1期153-160,共8页
Journal of Guangdong Ocean University
基金
湛江市科技计划项目(2022A01212)。
关键词
凡纳滨对虾
谷胱甘肽酵母水解物
干露胁迫
糖代谢
Litopenaeus vannamei
glutathione yeast hydrolysate
air exposure
glucose metabolism
