摘要
【目的】研究饲料牛磺酸添加水平对刺参(Apostichopus japonicus)生长、体组成、抗氧化能力以及消化代谢的影响,以探究幼刺参的牛磺酸合成能力及对牛磺酸的需求量。【方法】选用初始体质量为(11.40±0.04)g的刺参为研究对象,在基础饲料中分别添加0(对照)、0.30%、0.60%、0.90%、1.20%和1.50%的包被牛磺酸以及0.60%半胱氨酸和0.60%磺基丙氨酸(均以质量分数计,下同),形成8组等氮等脂的实验饲料,在养殖桶(400 L)内进行喂养实验。每种饲料投喂3桶幼参(每桶30头),日投喂1次,实验周期56 d,测定刺参生长性能、抗氧化、代谢酶活性指标。【结果】添加0.30%~1.50%的牛磺酸显著提高了幼参末质量、增重率和特定生长率(P<0.05),而添加0.60%~1.50%的牛磺酸显著提高了刺参的粪便产生率(P<0.05)。与对照组相比,饲料中添加0.60%~1.20%牛磺酸显著提高了全参粗蛋白含量,添加1.20%和1.50%牛磺酸显著提高全参灰分含量(P<0.05)。随饲料中牛磺酸添加量的梯度升高,全参苏氨酸、缬氨酸、蛋氨酸以及半胱氨酸等氨基酸含量呈现上升趋势而组氨酸含量则呈现下降趋势,亮氨酸则呈现先上升后降低的趋势,添加量0.90%时平均值最高,显著高于对照组(P<0.05)。与对照组相比,添加0.60%~1.50%牛磺酸显著提高体壁和呼吸树中牛磺酸含量,添加0.30%~1.50%牛磺酸显著提高肠道牛磺酸(P<0.05)。磺基丙氨酸添加组肠道牛磺酸含量显著高于对照组,但是显著低于1.20%~1.50%牛磺酸添加组(P<0.05)。随饲料中牛磺酸添加量升高,刺参肠道脂肪酶、淀粉酶和丙酮酸激酶等活性呈先升高后下降趋势,且均在0.90%牛磺酸添加组达到最大值;所有牛磺酸添加组刺参肠道的葡萄糖激酶、碱性磷酸酶等酶活性均高于对照组(P<0.05)。半胱亚磺酸脱羧酶和半胱氨酸双加氧酶活性呈逐渐下降的趋势,且饲料中添加0.60%~1.50%牛磺酸显著降低了肠道半胱亚磺酸�
【Objective】To investigate the taurine synthesis ability of junvenile sea cucumber(Apostichopus japonicus)and its requirement for dietary taurine by analyzing the effects of dietary taurine on growth,body composition,antioxidation and digestion.【Method】Sea cucumber with initial weight of(11.40±0.04)g was used in the experimentexperiment.0(control),0.30%,0.60%,0.90%,1.20%,and 1.50%coated taurine as well as 0.60%cysteine and 0.60%sulfoalanine were added to the basic feed to form 8 isonitrogenous and isolipdic diets.The experiment was conducted in a tank(400 L),and each diet was put into three replicate tanks(30 per tank)once a day.The experiment lasted 56 days.The indicators of growth performance,antioxidant and metabolic enzyme activity of sea cucumber were measured.【Result】At the end of experiment,diets with 0.30%-1.50%taurine significantly increased the final weight,weight gain,and specific growth rate,while diets with 0.60%-1.50%taurine significantly increased the feces production rate(P<0.05).The ash content of whole body in groups of 1.20%and 1.50%taurine and the protein content of whole body in groups of 0.60%,0.90%and 1.20%taurine were significantly higher than those in the control group(P<0.05).As taurine level increased,the contents of threonine,valine,methionine,phenylalanine,glycine,cysteine,and proline increased while histidine and aspartic acid contents decreased.Leucine content first increased and then decreased,and the mean content in group of 0.90%taurine is the highest with significant difference(P<0.05)from the control group.The taurine content in respiratory tree and intestine gradually increased and then plateaued.Diets with 0.60%-1.50%taurine significantly increased taurine content of body wall,and all taurine treatments significantly increased intestine taurine content(P<0.05).The intestinal taurine content was significantly higher in sulfonylalanine group than that of the control group,but lower than those fed 1.20%-1.50%taurine(P<0.05).The activities of intestinal lipase,amylase
作者
李培玉
王美琪
宋志东
胡顺鑫
刘财礼
李璐
刘经未
陆国峰
LI Peiyu;WANG Meiqi;SONG Zhidong;HU Shunxin;LIU Caili;LI Lu;LIU Jingwei;LU Guofeng(Shandong Marine Resource and Environment Research Institute,Yantai 264006,China;Shanghai Ocean University National Demonstration Center for Experimental Fisheries Science Education,Shanghai 201306,China)
出处
《广东海洋大学学报》
CAS
CSCD
北大核心
2024年第1期9-19,共11页
Journal of Guangdong Ocean University
基金
山东省重点研发计划(2020CXGC011404)
山东省现代农业产业技术体系刺参产业技术体系(SDAIT-22-06)。
关键词
刺参
牛磺酸
生长
消化代谢
抗氧化
Apostichopus japonicus
taurine
growth
digestion and metabolism
antioxidation
