摘要
【目的】2023年6月在湖南省永州市道县仙子脚镇多个村的水稻上发现一种叶条斑病,发生面积超过30 hm^(2),为探究发病原因,开展相关研究。【方法】利用组织分离法对道县采集的水稻病叶进行病原菌分离,对分离到的病菌进行形态学观察,结合ITS、TEF1-α和TUB2基因序列联合建树,确定病原物,利用柯赫氏法则进行病原物验证。【结果】湖南省永州市道县仙子脚镇发生的水稻叶条斑病病原菌为稻黑孢(Nigrospora oryzae)。【结论】明确了湖南省道县水稻叶条斑病的病原菌,为今后防控工作的开展提供参考。
[Objective]In June 2023,a new leaf stripe disease was discovered on rice in some villages of Xianzijiao Town,Daoxian County,Yongzhou City,Hunan Province,with an area of over 30 hm^(2).To investigate the cause of the disease,relevant researches were conducted.[Method]The pathogen was isolated from diseased rice leaves collected from Daoxian County by using the tissue isolation method.Morphological observation combined with ITS,TEF1-αand TUB2 gene sequence evolutionary tree were used to identify the pathogen,and then the pathogen was validated by the Koch’s rule.[Result]The pathogen of the rice streak leaf spots was identified as Nigrospora oryzae.[Conclusion]The pathogen of the rice leaf stripe disease was identified,providing references for the prevention and control of the disease in the future.
作者
王潇琪
任晓凤
杨华
何斌
邓义昌
周倩
WANG Xiaoqi;REN Xiaofeng;YANG Hua;HE Bin;DENG Yichang;ZHOU Qian(Hunan Provincial Key Laboratory of Plant Disease Control and Utilization/College of Plant Protection,Hunan Agricultural University,Changsha 410125,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410125,China;Bureau of Agriculture and Rural Affairs of Daoxian County,Yongzhou,Hunan 425399,China;Comprehensive Agricultural Service Center of Daoxian County,Yongzhou,Hunan 425399,China)
出处
《生物灾害科学》
2024年第1期28-33,共6页
Biological Disaster Science
基金
农业农村部农业外来入侵物种发生危害及扩散风险调查项目(13220146)。
关键词
水稻叶条斑病
分离鉴定
柯赫氏法则
稻黑孢菌
rice leaf stripe disease
isolation and identification
Koch’s rule
Nigrospora oryzae
