摘要
为了探究不同砧木嫁接花椒果皮中非挥发性成分的差异,以梅花椒为接穗品种,以川陕花椒(YJ)、七月椒(QJ)、八月椒(BJ)为砧木品种,同时将实生繁育梅花椒作为对照(MJ)。利用超高效液相色谱—串联质谱(UPLC-MS/MS)的代谢组学技术结合多元统计分析,分别比较了3种砧木嫁接梅花椒与对照果皮中非挥发性成分。结果表明,4个品种的花椒果皮中共检测出12类1223种非挥发性成分,主要包括黄酮、酚酸类、脂质、生物碱、氨基酸及其衍生物等。与对照相比,3种砧木(YJ,QJ,BJ)嫁接下的梅花椒分别有16、56、78种差异代谢物。YJ更加接近对照材料;QJ中氨基酸及其衍生物、酚酸类、黄酮类代谢物积累更多,使其在药用价值提高的同时,营养价值也得到了改善;BJ在不考虑其药用和食用价值的前提下,在应对逆境胁迫方面可能具有开发潜力。本研究结果可为花椒的果皮品质分析及嫁接苗繁育提供一定的参考。
In order to explore the difference of non-volatile components in the pericarp of Zanthoxylum bungeanum grafted with different rootstocks.Meihuajiao as the scion variety,and three rootstock varieties with Chuanshanhua jiao(YJ),Qiyuejiao(QJ),and Bayuejiao(BJ),at the same time seedlings of Meihuajiao were compared as control(MJ).Using UPLC-MS/MS metabonomics combined with multivariate statistical analysis,the non-volatile com ponents of three rootstocks grafted varieties and the control were compared respectively.The results showed that there were 12 categories of 1223 non-volatile components in the pericarp of four varieties of Z.bungeanum,includ ing flavonoids,phenolic acids,lipids,alkaloids,amino acids and their derivatives,etc.Compared with the control,there were 16,56,and 78 differential metabolites in the grafted varieties from the three rootstocks(YJ,QJ,BJ).Among them,YJ as rootstock is closer to the control.When QJ was used as rootstock,amino acids and their derivatives,phenolic acids and flavonoid metabolites accumulated more,which not only increased its medicinal value,but also improved its nutritional value.Taking BJ as rootstock,without considering its medicinal and edible value,may have development potential in coping with adversity stress.The results of this study can provide some reference for the improving the pericarp quality and breeding of Z.bungeanum.
作者
武衡
曹永红
张春回
任苗
王勃
宫盱彤
Wu Heng;Cao Yonghong;Zhang Chunhui;Ren Miao;Wang Bo;Gong Xutong(Institute of Chinese Red Pepper,Longnan Academy of Economic Forest,Longnan,746000)
出处
《分子植物育种》
CAS
北大核心
2024年第5期1600-1612,共13页
Molecular Plant Breeding
基金
中央林业科技推广示范项目([2020]ZYTG07号)
甘肃省科技计划项目(20YF8NK161)
陇原青年创新创业人才个人项目(2021LQGR42)共同资助。
