摘要
化学或物理因子作为诱导物,能够直接或间接影响菌体基因转录表达。为探索高级醇底物对白地霉的诱导效果,以分离自土壤的白地霉S12为对象,采用五种高级醇(正丙醇、正丁醇、异丁醇、正己醇和异戊醇)作为诱导物,研究不同高级醇的诱导剂量、诱导时间对菌体S12降解不同高级醇活性及脱氢酶活力的影响。结果表明,当正己醇和异丁醇分别作为诱导剂时,胞内酶活力较高。正丙醇、正丁醇、异丁醇和正己醇作为诱导剂时,最适诱导时间均为6 h;而以异戊醇为诱导剂时,最佳诱导时间为12 h。以正丙醇和正己醇为诱导剂时,最适的诱导浓度为1.5 g/L;以正丁醇、异丁醇和异戊醇为诱导剂时,最适诱导浓度分别为1.0、0.5和2.5 g/L。NativePAGE电泳结果表明,以五种高级醇为诱导剂均能使菌体合成分子量为223 kDa左右的脱氢酶。其中,以正己醇为诱导剂时所得菌体同时降解多种高级醇的能力最强,其降解产物均为其相应的酸类和酯类物质。
The inducers of the chemical or physical factors could affect the gene expression and transcription of strain directly or indirectly.In order to explore the inductive effect of higher alcohol on Galactomyces geotrichum,the G.geotrichum S12,derived from the soil,was induced by five higher alcoh ols,including n-propanol,n-butanol,isobutanol,nhexanol and isoamyl alcohol.The effects of induction dose and induction time for degradation activit y of different higher alcohols by strain S12 and its transforming enzyme were studi ed.The results showed that the enzyme activity formed by strain S12 was higher when the inducers were chosen as n-hexanol and isobutanol.The optimum induction time was 6 h when n-propanol,n-butanol,isobutanol and n-hexanol were used as inducers.While the optimum induction ti me of strain S12 and enzyme induced by isoamyl alcohol was 12 h.When n-propanol and n-hexanol were used as inducers,the optimal concentration was 1.5 g/L.While the optimal concentrations of strain S12 and enzyme induced by n-butanol,isobutanol and isoamyl alcohol were 1.0,0.5 and 2.5 g/L,respectively.The results of native polyacryplamide gel electrophoresis(Native-PAGE)indicated that dehydrogenase formation,molecular weight about 223 kDa,was induced by five higher alcohols.In particular,the strain,induced by hexanol,had much higher c apability in degrading five higher alcohols at the same time.The products of the above five higher alcohols after catalyzing by G.geotrichum S12 induced by hexanol were their corresponding acids and esters.
作者
朱静
杨晓聪
陈亚蓝
刘海波
陈龙
郑雪珂
师俊玲
ZHU Jing;YANG Xiaocong;CHEN Yalan;LIU Haibo;CHEN Long;ZHENG Xueke;SHI Junling(College of Food Science and Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China;School of Life Sciences,Northwestern Polytechnical University,Xi’an 710072,China)
出处
《食品工业科技》
CAS
北大核心
2024年第6期128-141,共14页
Science and Technology of Food Industry
基金
河南省重点研发与推广专项(科技攻关)项目(212102110314)
河南省高等学校青年骨干教师培养计划(2019GGJS264)
河南省青年科学基金项目(212300410228)
河南省本科高校研究性教学改革研究与实践项目(2022SYJXLX119)
信阳农林学院2021年校级教育教学改革研究与实践项目(2021XJGLX21)
信阳农林学院高水平科研孵化器建设基金项目(FCL202014)
信阳农林学院2019年度学校青年基金(2019LG009)。
关键词
白地霉
高级醇脱氢酶
诱导物
酶活性
诱导条件
Galactomyces geotrichum
higher alcohol dehydrogenase
inducer
enzyme activity
induction conditions
