摘要
目的 比较被动超声冲洗技术、EDDY声波冲洗技术和Er∶YAG激光冲击波增强型发射光声流(shock-wave enhanced emission photo acoustic streaming,SWEEPS)模式冲洗技术清除根管内粪肠球菌的效果。方法 本研究于2023年2—6月在上海交通大学医学院附属第九人民医院牙体牙髓科及口腔微生态与系统性疾病实验室进行。构建3D下颌第一前磨牙粪肠球菌根管感染牙齿模型15颗,将模型分为超声组、EDDY组、SWEEPS-Er∶YAG组,每组5颗。应用上述相应冲洗方式对3组牙齿模型进行根管冲洗,每颗牙齿模型冲洗3次,冲洗前及每次冲洗后用无菌纸尖采集主根管和侧支根管样本,用平板细菌计数法对样本进行检测分析。结果 SWEEPS-Er∶YAG组主根管在冲洗30、60 s后和侧支根管在冲洗60、90 s后菌落数均显著小于超声组和EDDY组,差异均有统计学意义(均P <0.05);SWEEPS-Er∶YAG组主根管在冲洗30、60 s后和侧支根管在冲洗30、60、90 s后细菌载量的减少率均高于超声组和EDDY组,差异均有统计学意义(均P <0.05)。结论 Er∶YAG激光SWEEPS模式冲洗技术较被动超声冲洗技术、EDDY声波冲洗技术可更快更好地清除根管内粪肠球菌。
Objective To compare the effect of ultrasonic activated irrigation,EDDY irrigation and Er∶YAG laser shock-wave enhanced emission photo acoustic streaming(SWEEPS)on the removal of Enterococcus faecalis in root canals.Methods This study was carried out in the Department of Endodontics and the Laboratory of Oral Microecology and Systemic Diseases,the Ninth People′s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from February to June 2023.Fifteen root canal models of mandibular first premolars infected with Enterococcus faecalis were constructed and divided into ultrasound group,EDDY group,and SWEEPS-Er∶YAG group,with five models in each group.The root canals of three groups of tooth models were irrigated with the corresponding irrigation methods mentioned above,each tooth model was rinsed 3 times,and the samples of main root canals and lateral root canals were collected with sterile paper before and after each rinse,and the samples were detected and analyzed by plate bacterial count method.Results SWEEPS-Er∶YAG group had significantly fewer colonies in the main root canal at 30 s and 60 s after irrigation and in the lateral root canal at 60 s and 90 s after irrigation than ultrasonic group and EDDY group(all P<0.05).The reduction rate of bacterial load in the main root canal at 30 s and 60 s afterirrigation and in the lateral root canal at 30 s,60 s and 90 s after irrigation in SWEEPS-Er∶YAG group was significantly higher than that in ultrasonic group and EDDY group(all P<0.05).Conclusion Er∶YAG laser SWEEPS mode can remove Enterococcus faecalis in root canals faster and better than passive ultrasonic activated irrigation and EDDY irrigation.
作者
陈燕
张彭飞
苑克勇
黄正蔚
CHEN Yan;ZHANG Peng-fei;YUAN Ke-yong;HUANG Zheng-wei(National Center for Stomatology,National Center for Clinical Research of Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Institute of Stomatology,Department of Nursing&Department of Endodontics,the Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200011,China)
出处
《中国实用口腔科杂志》
CAS
CSCD
2024年第1期62-66,共5页
Chinese Journal of Practical Stomatology
基金
上海交通大学医学院附属第九人民医院护理院级基金项目(YHL20213D04)
上海口腔研究型学科项目(KQYJXK2020)。